Document Detail

Role of the Rhesus glycoprotein, Rh B glycoprotein, in renal ammonia excretion.
MedLine Citation:
PMID:  20719974     Owner:  NLM     Status:  MEDLINE    
Rh B glycoprotein (Rhbg) is a member of the Rh glycoprotein family of ammonia transporters. In the current study, we examine Rhbg's role in basal and acidosis-stimulated acid-base homeostasis. Metabolic acidosis induced by HCl administration increased Rhbg expression in both the cortex and outer medulla. To test the functional significance of increased Rhbg expression, we used a Cre-loxP approach to generate mice with intercalated cell-specific Rhbg knockout (IC-Rhbg-KO). On normal diet, intercalated cell-specific Rhbg deletion did not alter urine ammonia excretion, pH, or titratable acid excretion significantly, but it did decrease glutamine synthetase expression in the outer medulla significantly. After metabolic acidosis was induced, urinary ammonia excretion was significantly less in IC-Rhbg-KO than in control (C) mice on days 2-4 of acid loading, but not on day 5. Urine pH and titratable acid excretion and dietary acid intake did not differ significantly between acid-loaded IC-Rhcg-KO and C mice. In IC-Rhbg-KO mice, acid loading increased connecting segment (CNT) cell and outer medullary collecting duct principal cell Rhbg expression. In both C and IC-Rhbg-KO mice, acid loading decreased glutamine synthetase in both the cortex and outer medulla; the decrease on day 3 was similar in IC-Rhbg-KO and C mice, but on day 5 it was significantly greater in IC-Rhbg-KO than in C mice. We conclude 1) intercalated cell Rhbg contributes to acidosis-stimulated renal ammonia excretion, 2) Rhbg in CNT and principal cells may contribute to renal ammonia excretion, and 3) decreased glutamine synthetase expression may enable normal rates of ammonia excretion under both basal conditions and on day 5 of acid loading in IC-Rhbg-KO mice.
Jesse M Bishop; Jill W Verlander; Hyun-Wook Lee; Raoul D Nelson; Arthur J Weiner; Mary E Handlogten; I David Weiner
Related Documents :
3610144 - Increased concentrations of various amino acids in schizophrenic patients. evidence for...
509404 - Urinary metabolite of 1-(2,4,6-trichlorophenyl)-3,3-dimethyltriazene with an intact dia...
5427484 - Paper chromatographic survey of urinary amino-acids in muscular dystrophy.
6875384 - Intestinal absorption, excretion, and biotransformation of hyodeoxycholic acid in man.
9779624 - Use of citric acid for neutralizing polymerase chain reaction inhibition by chlorogenic...
21347974 - Chemical constituents of talauma arcabucoana (magnoliaceae): their brine shrimp lethali...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-08-18
Journal Detail:
Title:  American journal of physiology. Renal physiology     Volume:  299     ISSN:  1522-1466     ISO Abbreviation:  Am. J. Physiol. Renal Physiol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-04     Completed Date:  2010-12-02     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  100901990     Medline TA:  Am J Physiol Renal Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  F1065-77     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acidosis / genetics,  metabolism
Acids / pharmacology
Ammonia / urine*
Bicarbonates / blood
Blotting, Western
Electrolytes / metabolism
Gene Deletion
Gene Expression Regulation, Enzymologic / physiology
Glutamate-Ammonia Ligase / biosynthesis
Glycoproteins / genetics,  metabolism*
Kidney / metabolism*
Membrane Transport Proteins / genetics,  metabolism*
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Potassium / blood
Grant Support
Reg. No./Substance:
0/Acids; 0/Bicarbonates; 0/Electrolytes; 0/Glycoproteins; 0/Membrane Transport Proteins; 0/RhBG protein, mouse; 7664-41-7/Ammonia; EC Ligase; RWP5GA015D/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Fibroblast growth factor-10 signals development of von Brunn's nests in the exstrophic bladder.
Next Document:  Adding a statin to a combination of ACE inhibitor and ARB normalizes proteinuria in experimental dia...