| Role of the Rhesus glycoprotein, Rh B glycoprotein, in renal ammonia excretion. | |
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MedLine Citation:
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PMID: 20719974 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rh B glycoprotein (Rhbg) is a member of the Rh glycoprotein family of ammonia transporters. In the current study, we examine Rhbg's role in basal and acidosis-stimulated acid-base homeostasis. Metabolic acidosis induced by HCl administration increased Rhbg expression in both the cortex and outer medulla. To test the functional significance of increased Rhbg expression, we used a Cre-loxP approach to generate mice with intercalated cell-specific Rhbg knockout (IC-Rhbg-KO). On normal diet, intercalated cell-specific Rhbg deletion did not alter urine ammonia excretion, pH, or titratable acid excretion significantly, but it did decrease glutamine synthetase expression in the outer medulla significantly. After metabolic acidosis was induced, urinary ammonia excretion was significantly less in IC-Rhbg-KO than in control (C) mice on days 2-4 of acid loading, but not on day 5. Urine pH and titratable acid excretion and dietary acid intake did not differ significantly between acid-loaded IC-Rhcg-KO and C mice. In IC-Rhbg-KO mice, acid loading increased connecting segment (CNT) cell and outer medullary collecting duct principal cell Rhbg expression. In both C and IC-Rhbg-KO mice, acid loading decreased glutamine synthetase in both the cortex and outer medulla; the decrease on day 3 was similar in IC-Rhbg-KO and C mice, but on day 5 it was significantly greater in IC-Rhbg-KO than in C mice. We conclude 1) intercalated cell Rhbg contributes to acidosis-stimulated renal ammonia excretion, 2) Rhbg in CNT and principal cells may contribute to renal ammonia excretion, and 3) decreased glutamine synthetase expression may enable normal rates of ammonia excretion under both basal conditions and on day 5 of acid loading in IC-Rhbg-KO mice. |
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Authors:
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Jesse M Bishop; Jill W Verlander; Hyun-Wook Lee; Raoul D Nelson; Arthur J Weiner; Mary E Handlogten; I David Weiner |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-08-18 |
Journal Detail:
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Title: American journal of physiology. Renal physiology Volume: 299 ISSN: 1522-1466 ISO Abbreviation: Am. J. Physiol. Renal Physiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-04 Completed Date: 2010-12-02 Revised Date: 2013-05-13 |
Medline Journal Info:
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Nlm Unique ID: 100901990 Medline TA: Am J Physiol Renal Physiol Country: United States |
Other Details:
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Languages: eng Pagination: F1065-77 Citation Subset: IM |
Affiliation:
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Div. of Nephrology, Hypertension, and Transplantation, P.O. Box 100224, Univ. of Florida College of Medicine, Gainesville, FL 32610-0224, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acidosis
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genetics,
metabolism Acids / pharmacology Alleles Ammonia / urine* Animals Bicarbonates / blood Blotting, Western Diet Electrolytes / metabolism Gene Deletion Gene Expression Regulation, Enzymologic / physiology Glutamate-Ammonia Ligase / biosynthesis Glycoproteins / genetics, metabolism* Immunohistochemistry Kidney / metabolism* Membrane Transport Proteins / genetics, metabolism* Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Potassium / blood |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK045788/DK/NIDDK NIH HHS; R01 DK045788-15/DK/NIDDK NIH HHS; R01-DK-45788/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acids; 0/Bicarbonates; 0/Electrolytes; 0/Glycoproteins; 0/Membrane Transport Proteins; 0/RhBG protein, mouse; 7440-09-7/Potassium; 7664-41-7/Ammonia; EC 6.3.1.2/Glutamate-Ammonia Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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