Document Detail


Role of RYR3 splice variants in calcium signaling in mouse nonpregnant and pregnant myometrium.
MedLine Citation:
PMID:  17596299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alternative splicing of ryanodine receptor subtype 3 (RYR3) may generate a short isoform (RYR3S) without channel function and a functional full-length isoform (RYR3L). The RYR3S isoform has been shown to negatively regulate the native RYR2 subtype in smooth muscle cells as well as the RYR3L isoform when both isoforms were coexpressed in HEK-293 cells. Mouse myometrium expresses only the RYR3 subtype, but the role of RYR3 isoforms obtained by alternative splicing and their activation by cADP-ribose during pregnancy have never been investigated. Here, we show that both RYR3S and RYR3L isoforms are differentially expressed in nonpregnant and pregnant mouse myometrium. The use of antisense oligonucleotides directed against each isoform indicated that only RYR3L was activated by caffeine and cADP-ribose in nonpregnant myometrium. These RYR3L-mediated Ca(2+) releases were negatively regulated by RYR3S expression. At the end of pregnancy, the relative expression of RYR3L versus RYR3S and its ability to respond to cADP-ribose were increased. Therefore, our results suggest that physiological regulation of RYR3 alternative splicing may play an essential role at the end of pregnancy.
Authors:
Fabrice Dabertrand; Nicolas Fritz; Jean Mironneau; Nathalie Macrez; Jean-Luc Morel
Related Documents :
19144959 - Il11 antagonist inhibits uterine stromal differentiation, causing pregnancy failure in ...
3205209 - Maternal hyperthermia as a cause of "idiopathic" mental retardation.
16809609 - Coordination between ccr7- and ccr9-mediated chemokine signals in prevascular fetal thy...
20656339 - Pregnancy-associated glycoprotein (pag) family localized in chorionic cells within the ...
18325889 - Villous explant culture using early gestation tissue from ongoing pregnancies with know...
16375819 - Immunohistochemistry of adhesion molecules, metalloproteinases and no-synthases in extr...
2312189 - Value of sperm characteristics and the result of in-vitro fertilization for predicting ...
7988439 - Identification of relaxin gene expression and protein localization in the uterine endom...
24722709 - Adenomyosis in pregnancy mimicking morbidly adherent placenta.
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2007-06-27
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  293     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-12     Completed Date:  2007-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C848-54     Citation Subset:  IM    
Affiliation:
Centre de Neurosciences Intégratives et Cognitives, UMR5228 CNRS, Université Bordeaux 1 and Université Bordeaux 2, Ave. des Facultés, Talence 33405, France. jlucmorel.cnic@voila.fr
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alternative Splicing / physiology
Animals
Caffeine / pharmacology
Calcium / metabolism
Calcium Signaling / drug effects,  physiology*
Cyclic ADP-Ribose / metabolism
Female
Gene Expression Regulation / physiology
Mice
Myocytes, Smooth Muscle / cytology,  metabolism*
Myometrium / cytology*
Oligonucleotides, Antisense / pharmacology
Phosphodiesterase Inhibitors / pharmacology
Pregnancy
Pregnancy, Animal / physiology*
Ryanodine Receptor Calcium Release Channel / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Oligonucleotides, Antisense; 0/Phosphodiesterase Inhibitors; 0/Ryanodine Receptor Calcium Release Channel; 119340-53-3/Cyclic ADP-Ribose; 58-08-2/Caffeine; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Contribution of KCNQ1 to the regulatory volume decrease in the human mammary epithelial cell line MC...
Next Document:  Paxillin phosphorylation, actin polymerization, noise temperature, and the sustained phase of swine ...