Document Detail

Role of RYR3 splice variants in calcium signaling in mouse nonpregnant and pregnant myometrium.
MedLine Citation:
PMID:  17596299     Owner:  NLM     Status:  MEDLINE    
Alternative splicing of ryanodine receptor subtype 3 (RYR3) may generate a short isoform (RYR3S) without channel function and a functional full-length isoform (RYR3L). The RYR3S isoform has been shown to negatively regulate the native RYR2 subtype in smooth muscle cells as well as the RYR3L isoform when both isoforms were coexpressed in HEK-293 cells. Mouse myometrium expresses only the RYR3 subtype, but the role of RYR3 isoforms obtained by alternative splicing and their activation by cADP-ribose during pregnancy have never been investigated. Here, we show that both RYR3S and RYR3L isoforms are differentially expressed in nonpregnant and pregnant mouse myometrium. The use of antisense oligonucleotides directed against each isoform indicated that only RYR3L was activated by caffeine and cADP-ribose in nonpregnant myometrium. These RYR3L-mediated Ca(2+) releases were negatively regulated by RYR3S expression. At the end of pregnancy, the relative expression of RYR3L versus RYR3S and its ability to respond to cADP-ribose were increased. Therefore, our results suggest that physiological regulation of RYR3 alternative splicing may play an essential role at the end of pregnancy.
Fabrice Dabertrand; Nicolas Fritz; Jean Mironneau; Nathalie Macrez; Jean-Luc Morel
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2007-06-27
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  293     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-12     Completed Date:  2007-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C848-54     Citation Subset:  IM    
Centre de Neurosciences Intégratives et Cognitives, UMR5228 CNRS, Université Bordeaux 1 and Université Bordeaux 2, Ave. des Facultés, Talence 33405, France.
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MeSH Terms
Alternative Splicing / physiology
Caffeine / pharmacology
Calcium / metabolism
Calcium Signaling / drug effects,  physiology*
Cyclic ADP-Ribose / metabolism
Gene Expression Regulation / physiology
Myocytes, Smooth Muscle / cytology,  metabolism*
Myometrium / cytology*
Oligonucleotides, Antisense / pharmacology
Phosphodiesterase Inhibitors / pharmacology
Pregnancy, Animal / physiology*
Ryanodine Receptor Calcium Release Channel / genetics*,  metabolism
Reg. No./Substance:
0/Oligonucleotides, Antisense; 0/Phosphodiesterase Inhibitors; 0/Ryanodine Receptor Calcium Release Channel; 119340-53-3/Cyclic ADP-Ribose; 58-08-2/Caffeine; 7440-70-2/Calcium

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