Document Detail

Role of the RNA polymerase alpha subunit in transcription activation.
MedLine Citation:
PMID:  1484484     Owner:  NLM     Status:  MEDLINE    
The N-terminal two-thirds of the alpha subunit of Escherichia coli RNA polymerase plays an essential role in the initiation of subunit assembly, by gathering two large subunits, beta and beta', together into a core-enzyme complex. One group of RNA polymerase mutants deficient in response to transcription activation carries mutations in the C-terminal region of the alpha subunit, indicating that the C-terminal region of the alpha subunit is involved in protein-protein contact in positive control of transcription. A set of activators (class I transcription factors) which make contact with this contact site I region on RNA polymerase alpha subunit bind in most cases to DNA upstream of the promoter -35 signal. Genetic fine mapping indicates that a cluster of subsites exists in the contact site I region, each interacting with a set of the class I factors and each consisting of a structure formed by only 5-10 amino acid residues.
A Ishihama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Molecular microbiology     Volume:  6     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-02-17     Completed Date:  1993-02-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3283-8     Citation Subset:  IM    
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
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MeSH Terms
Bacterial Proteins / metabolism*
Binding Sites
DNA, Bacterial / metabolism
Escherichia coli / genetics*
Promoter Regions, Genetic
Protein Conformation
RNA Polymerase I / chemistry,  physiology*
Regulatory Sequences, Nucleic Acid
Transcription Factors / metabolism
Transcription, Genetic*
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/Transcription Factors; EC 2.7.7.-/RNA Polymerase I

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