Document Detail

Role of Neurokinin B and Dynorphin A in pituitary gonadotroph and somatolactotroph cell lines.
MedLine Citation:
PMID:  22641014     Owner:  NLM     Status:  MEDLINE    
The role of Neurokinin B (NKB) and Dynorphin A (Dyn) in the regulation of the hypothalamic pituitary axis is an important area of recent investigation. These peptides are critical for the rhythmic release of GnRH, which subsequently stimulates the secretion of the gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH). The present study utilized the gonadotroph cell line LβT2 and the somatolactotroph GH3 cell line to examine the possible role of these peptides in pituitary hormone secretion. The NKB receptor (NK3R) and the Dyn receptor (the κ-opiate receptor (KOR)) were both detected in LβT2 cells and GH3 cells. NKB, by itself, failed to increase gonadotropin LHβ and FSHβ promoter activities and did not modulate the effects of GnRH on gonadotropin promoter activity. In GH3 cells, NKB significantly increased TRH-induced PRL promoter activity although NKB alone did not have an effect on basal PRL promoter activity. Dyn had no effect on gonadotropin promoters alone or in combination with GnRH stimulation. PRL promoters stimulated by TRH were not significantly changed by Dyn. TRH-induced PRL promoter activity was further increased in the presence of higher concentrations of NKB, whereas Dyn did not have a significant effect on the PRL promoter even at a high concentration. In addition, TRH-induced ERK (Extracelluar signal-regulated kinase) activation was enhanced in the presence of NKB. Our current study demonstrated that NKB had a stimulatory effect on PRL expression in a PRL-producing cell, but had no effect on gonadotropin secretion from a gonadotroph cell line.
Tselmeg Mijiddorj; Haruhiko Kanasaki; Indri N Purwana; Aki Oride; Unurjargar Sukhbaatar; Kohji Miyazaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-28
Journal Detail:
Title:  Endocrine journal     Volume:  59     ISSN:  1348-4540     ISO Abbreviation:  Endocr. J.     Publication Date:  2012  
Date Detail:
Created Date:  2012-08-01     Completed Date:  2013-01-10     Revised Date:  2014-11-10    
Medline Journal Info:
Nlm Unique ID:  9313485     Medline TA:  Endocr J     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  631-40     Citation Subset:  IM    
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MeSH Terms
Cell Line
Dynorphins / genetics,  metabolism,  pharmacology,  physiology*
Gonadotrophs / drug effects,  metabolism*
Gonadotropins / genetics,  metabolism
Lactotrophs / drug effects,  metabolism*
Neurokinin B / genetics,  metabolism,  pharmacology,  physiology*
Pituitary Gland / cytology,  drug effects,  metabolism
Prolactin / genetics,  metabolism
Promoter Regions, Genetic / drug effects
Receptors, Opioid / genetics,  metabolism
Receptors, Tachykinin / genetics,  metabolism
Somatotrophs / drug effects,  metabolism*
Reg. No./Substance:
0/Gonadotropins; 0/Receptors, Opioid; 0/Receptors, Tachykinin; 0/neurokinin receptor 4; 0/nociceptin receptor; 74913-18-1/Dynorphins; 86933-75-7/Neurokinin B; 9002-62-4/Prolactin

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