| Role of NF-kappaB in flow-induced vascular remodeling. | |
| | |
MedLine Citation:
|
PMID: 19320561 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Vascular remodeling associated with increased blood flow involves reactive oxygen species (ROS)-dependent activation of matrix metalloproteinases (MMPs). To investigate the potential role of NF-kappaB in this process, human umbilical vein endothelial cells were subjected to different flow conditions during a 24-h period. Normal (15 dynes/cm(2)) and high (30 dynes/cm(2)) shear stress induced IkappaBalpha degradation and NF-kappaB p65 phosphorylation, and activated MMP-2 and MMP-9. These effects were blunted in cells incubated with the NF-kappaB inhibitor pyrrolidine dithio-carbamate (PDTC). In mice, creation of a carotid artery-jugular vein arteriovenous fistula (AVF) increased carotid blood flow sixfold, triggering the increase in carotid diameter from 459 +/- 8 microm (before AVF) to 531 +/- 13 and 669 +/- 21 microm (7 and 21 days after AVF). ROS production and NF-kappaB activity were enhanced in fistulated carotids, but only the latter was blocked by PDTC, although PDTC blocked ROS production in vitro. In PDTC-treated mice, changes in carotid caliber and shear stress matched controls at 7 days, but carotids enlarged only marginally thereafter, reaching only 578 +/- 8 microm at 21 days (p < 0.01 vs. untreated). Similarly, both MMP-9 expression and activity were abrogated by PDTC at 3 weeks. Hence, induction of NF-kappaB by shear stress contributes to MMP induction and allows long-term flow-induced vascular enlargement. |
| | |
Authors:
|
Yves Castier; Bhama Ramkhelawon; Stéphanie Riou; Alain Tedgui; Stephanie Lehoux |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Antioxidants & redox signaling Volume: 11 ISSN: 1557-7716 ISO Abbreviation: Antioxid. Redox Signal. Publication Date: 2009 Jul |
Date Detail:
|
Created Date: 2009-12-01 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100888899 Medline TA: Antioxid Redox Signal Country: United States |
Other Details:
|
Languages: eng Pagination: 1641-9 Citation Subset: IM |
Affiliation:
|
Parts Cardiovascular Research Center, Inserm U970, HEGP, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Increased prevalence of insulin resistance in patients with differentiated thyroid carcinoma.
Next Document: Hemodynamic forces, vascular oxidative stress and regulation of BMP-2/4 expression.