Document Detail

Role of NAD(P)H:quinone oxidoreductase encoded by drgA gene in reduction of exogenous quinones in cyanobacterium Synechocystis sp. PCC 6803 cells.
MedLine Citation:
PMID:  15000679     Owner:  NLM     Status:  MEDLINE    
Insertion mutant Ins2 of the cyanobacterium Synechocystis sp. PCC 6803, lacking NAD(P)H:quinone oxidoreductase (NQR) encoded by drgA gene, was characterized by higher sensitivity to quinone-type inhibitors (menadione and plumbagin) than wild type (WT) cells. In photoautotrophically grown cyanobacterial cells more than 60% of NADPH:quinone-reductase activity, as well as all NADPH:dinoseb-reductase activity, was associated with the function of NQR. NQR activity was observed only in soluble fraction of cyanobacterial cells, but not in membrane fraction. The effects of menadione and menadiol on the reduction of Photosystem I reaction center (P700(+)) after its photooxidation in the presence of DCMU were studied using the EPR spectroscopy. The addition of menadione increased the rate of P700(+) reduction in WT cells, whereas in Ins2 mutant the reduction of P700(+) was strongly inhibited. In the presence of menadiol the reduction of P700(+) was accelerated both in WT and Ins2 mutant cells. These data suggest that NQR protects the cyanobacterial cells from the toxic effect of exogenous quinones by their reduction to hydroquinones. These data may also indicate the probable functional homology of Synechocystis sp. PCC 6803 NQR with mammalian and plant NAD(P)H:quinone oxidoreductases (DT-diaphorases).
I V Elanskaya; V G Grivennikova; V V Groshev; G V Kuznetsova; M E Semina; K N Timofeev
Related Documents :
19640839 - Role of mammalian ecdysoneless in cell cycle regulation.
11394439 - The quartz crystal microbalance as a novel means to study cell-substrate interactions i...
21511259 - On the electrophysiological response of bone cells using a stokesian fluid stimulus pro...
15000679 - Role of nad(p)h:quinone oxidoreductase encoded by drga gene in reduction of exogenous q...
2272319 - Growth control in mammalian cells by cell-cell contacts.
18812319 - Isolation of hyperactive mutants of mammalian target of rapamycin.
23600329 - Restoration of igfbp-rp1 increases radiosensitivity and chemosensitivity in hormone-ref...
7471759 - Ultrastructural investigation of renomedullary interstitial cells in "endocrine kidney".
17850209 - Structure and function of the cell surface (tethered) mucins.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry. Biokhimii͡a     Volume:  69     ISSN:  0006-2979     ISO Abbreviation:  Biochemistry Mosc.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-03-05     Completed Date:  2005-01-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376536     Medline TA:  Biochemistry (Mosc)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  137-42     Citation Subset:  IM    
Department of Genetics, Faculty of Biology, Lomonosov Moscow State University, Moscow 119992, Russia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacterial Proteins / genetics,  metabolism*
Chlorophyll / metabolism*
Cyanobacteria / enzymology*,  genetics
Drug Resistance, Bacterial / genetics
Mutagenesis, Insertional
NAD(P)H Dehydrogenase (Quinone) / genetics,  metabolism*
Oxidation-Reduction / drug effects
Photosystem I Protein Complex / drug effects,  metabolism
Quinones / metabolism,  pharmacology
Vitamin K 3 / metabolism*,  pharmacology
Reg. No./Substance:
0/Bacterial Proteins; 0/Photosystem I Protein Complex; 0/Quinones; 1406-65-1/Chlorophyll; 53321-11-2/chlorophyll P 700; 58-27-5/Vitamin K 3; EC Dehydrogenase (Quinone)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Interaction of HIV-1 reverse transcriptase with modified oligonucleotide primers containing 2'-O-bet...
Next Document:  Role of low-molecular-weight substrates in functional binding of the tRNAPhe acceptor end by phenyla...