| Role of NAD(P)H oxidase on vascular alterations in angiotensin II-infused mice. | |
| | |
MedLine Citation:
|
PMID: 15076159 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: Angiotensin (Ang) II stimulates vascular reactive oxygen species generation via NAD(P)H oxidase activation. We investigated whether vascular NAD(P)H oxidase influences structure and function of resistance arteries from Ang II-infused mice. METHODS: Mice received Ang II alone (400 ng/kg per min, subcutaneously), Ang II + apocynin (NAD(P)H oxidase inhibitor, 2.5 mg/day, in the food), apocynin alone or Ang II + hydralazine (50 mg/kg per day) for 14 days. Systolic blood pressure (SBP) was measured by tail-cuff methodology and function and structure of small mesenteric arteries were studied in pressurized vessels. Vascular collagen type I/III content was evaluated by confocal immunofluorescence microscopy and by immunoblotting. RESULTS: The rise in SBP induced by Ang II (P < 0.001) was prevented by apocynin and hydralazine. Media/lumen ratio increase in Ang II-infused mice (P < 0.01) was prevented by apocynin. Acetylcholine-mediated relaxation, which was impaired in Ang II-infused mice (P < 0.001), was improved by apocynin. Confocal microscopy and immunoblotting demonstrated increased collagen type I/III content in mesenteric arteries from Ang II-infused mice. Apocynin, but not hydralazine, prevented the increase in collagen abundance in Ang II-infused mice. The increase in vascular NAD(P)H oxidase activity by Ang II (P < 0.001) was prevented by apocynin. CONCLUSIONS: The NAD(P)H oxidase inhibitor apocynin reduced blood pressure elevation and prevented structural alterations, endothelial dysfunction, and collagen deposition in the media of small arteries in Ang II-infused mice. Although hydralazine also decreased blood pressure, it had no effects on vascular collagen content. Our findings suggest that NAD(P)H oxidase activity plays an important role in vascular functional and structural changes and in the composition of the vascular wall in Ang II-dependent hypertension. |
| | |
Authors:
|
Agostino Virdis; Mario Fritsch Neves; Farhad Amiri; Rhian M Touyz; Ernesto L Schiffrin |
Related Documents
:
|
11408379 - Role of brain angiotensin ii on somatosensory-induced antinatriuresis in hypertensive r... 12390049 - Does the angiotensin ii receptor antagonist losartan improve cognitive function? 8897949 - Control of renal and mesenteric conductance during hemorrhage in conscious hypertensive... 18156169 - Genetically altered animal models for mas and angiotensin-(1-7). 7664219 - Cardiac renin-angiotensin system: role in development of pressure-overload hypertrophy. 8583479 - Discovery of losartan, the first angiotensin ii receptor antagonist. 20401939 - Magnetic-field- and pressure-induced quantum phases in complex materials. 23267239 - Central diastolic blood pressure is associated with the degree of coronary collateral d... 3916779 - Neurologic intensive care unit monitoring. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of hypertension Volume: 22 ISSN: 0263-6352 ISO Abbreviation: J. Hypertens. Publication Date: 2004 Mar |
Date Detail:
|
Created Date: 2004-04-12 Completed Date: 2004-10-18 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8306882 Medline TA: J Hypertens Country: England |
Other Details:
|
Languages: eng Pagination: 535-42 Citation Subset: IM |
Affiliation:
|
CIHR Multidisciplinary Research Group on Hypertension, Clinical Research Institute of Montreal, University of Montreal, Quebec, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetophenones
/
pharmacology Angiotensin II / pharmacology* Animals Blood Pressure / drug effects, physiology Body Weight Collagen / metabolism Endothelium, Vascular / metabolism Enzyme Inhibitors / pharmacology Hydralazine / pharmacology Hypertension / metabolism, physiopathology* Male Mesenteric Arteries / physiology Mice Mice, Inbred BALB C NADPH Oxidase / antagonists & inhibitors, metabolism* Vascular Resistance / drug effects*, physiology* Vasoconstrictor Agents / pharmacology* Vasodilator Agents / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Acetophenones; 0/Enzyme Inhibitors; 0/Vasoconstrictor Agents; 0/Vasodilator Agents; 11128-99-7/Angiotensin II; 498-02-2/acetovanillone; 86-54-4/Hydralazine; 9007-34-5/Collagen; EC 1.6.3.1/NADPH Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of chronic nitric oxide synthase inhibition on cerebral arterioles in Wistar-Kyoto rats.
Next Document: Excessive zinc intake elevates systemic blood pressure levels in normotensive rats--potential role o...