Document Detail

Role of the N-terminal amino acid of Bacillus anthracis lethal factor in lethal toxin cytotoxicity and its effect on the lethal toxin neutralization assay.
MedLine Citation:
PMID:  18815235     Owner:  NLM     Status:  MEDLINE    
The cytotoxic activity of lethal factor (LF), a critical reagent used in the cell-based lethal toxin neutralization assay to assess anthrax vaccines, was shown to depend on the identity of its N-terminal amino acid, which plays a role in the targeting of LF to the proteasome for degradation. These results demonstrate that care must be taken to ensure that LF preparations used in standardized cell-based assays are not altered at their N-terminal ends.
Anita Verma; Leslie Wagner; Scott Stibitz; Nga Nguyen; Flor Guerengomba; Drusilla L Burns
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2008-09-24
Journal Detail:
Title:  Clinical and vaccine immunology : CVI     Volume:  15     ISSN:  1556-679X     ISO Abbreviation:  Clin. Vaccine Immunol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-06     Completed Date:  2008-12-01     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  101252125     Medline TA:  Clin Vaccine Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1737-41     Citation Subset:  IM    
Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
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MeSH Terms
Amino Acid Sequence
Amino Acid Substitution
Antigens, Bacterial / genetics,  toxicity*
Bacterial Toxins / genetics,  toxicity*
Cell Line
Macrophages / drug effects*
Neutralization Tests
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Toxins; 0/anthrax toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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