Document Detail


The role of membrane stiffness and actin turnover on the force exerted by DRG lamellipodia.
MedLine Citation:
PMID:  22713560     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We used optical tweezers to analyze the effect of jasplakinolide and cyclodextrin on the force exerted by lamellipodia from developing growth cones (GCs) of isolated dorsal root ganglia (DRG) neurons. We found that 25 nM of jasplakinolide, which is known to inhibit actin filament turnover, reduced both the maximal exerted force and maximal velocity during lamellipodia leading-edge protrusion. By using atomic force microscopy, we verified that cyclodextrin, which is known to remove cholesterol from membranes, decreased the membrane stiffness of DRG neurons. Lamellipodia treated with 2.5 mM of cyclodextrin exerted a larger force, and their leading edge could advance with a higher velocity. Neither jasplakinolide nor cyclodextrin affected force or velocity during lamellipodia retraction. The amplitude and frequency of elementary jumps underlying force generation were reduced by jasplakinolide but not by cyclodextrin. The action of both drugs at the used concentration was fully reversible. These results support the notion that membrane stiffness provides a selective pressure that shapes force generation, and confirm the pivotal role of actin turnover during protrusion.
Authors:
Ladan Amin; Erika Ercolini; Rajesh Shahapure; Elisa Migliorini; Vincent Torre
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-05
Journal Detail:
Title:  Biophysical journal     Volume:  102     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-20     Completed Date:  2012-10-17     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2451-60     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Affiliation:
Neuroscience Department, International School for Advanced Studies (SISSA), Trieste, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
Biomechanics / drug effects
Cell Membrane / drug effects,  physiology*
Cyclodextrins / pharmacology
Depsipeptides / pharmacology
Elastic Modulus / drug effects
Ganglia, Spinal / cytology*,  drug effects,  physiology*
Pseudopodia / drug effects,  physiology*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Actins; 0/Cyclodextrins; 0/Depsipeptides; 102396-24-7/jasplakinolide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Analytical tools to distinguish the effects of localization error, confinement, and medium elasticit...
Next Document:  CaMKII?C slows [Ca]i decline in cardiac myocytes by promoting Ca sparks.