| The role of interleukin-1 in wound biology. Part II: In vivo and human translational studies. | |
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MedLine Citation:
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PMID: 20889944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In the accompanying paper, we demonstrate that genetic variation within Nalp1 could contribute to interstrain differences in wound chemokine production through altering the amount of interleukin (IL)-1 produced. We further investigate the role of IL-1 in incisional wound biology and its effect on wound chemokine production in vivo and whether this mechanism could be active in human subjects. METHODS: A well-characterized murine model of incisional wounding was used to assess the in vivo role of IL-1 in wound biology. The amount of 7 different cytokines/chemokines produced within an experimentally induced skin incision on a mouse paw and the nociceptive response was analyzed in mice treated with an IL-1 inhibitor. We also investigated whether human IL-1β or IL-1α stimulated the production of chemokines by primary human keratinocytes in vitro, and whether there was a correlation between IL-1β and chemokine levels in 2 experimental human wound paradigms. RESULTS: Administration of an IL-1 receptor antagonist to mice decreased the nociceptive response to an incisional wound, and reduced the production of multiple inflammatory mediators, including keratinocyte-derived chemokine (KC) and macrophage inhibitory protein (MIP)-1α, within the wounds. IL-1α and IL-1β stimulated IL-8 and GRO-α (human homologues of murine keratinocyte-derived chemokine) production by primary human keratinocytes in vitro. IL-1β levels were highly correlated with IL-8 in human surgical wounds, and at cutaneous sites of human ultraviolet B-induced sunburn injury. CONCLUSIONS: IL-1 plays a major role in regulating inflammatory mediator production in wounds through a novel mechanism; by stimulating the production of multiple cytokines and chemokines, it impacts clinically important aspects of wound biology. These data suggest that administration of an IL-1 receptor antagonist within the perioperative period could decrease postsurgical wound pain. |
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Authors:
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Yajing Hu; Deyong Liang; Xiangqi Li; Hong-Hsing Liu; Xun Zhang; Ming Zheng; David Dill; Xiaoyou Shi; Yanli Qiao; David Yeomans; Brendan Carvalho; Martin S Angst; J David Clark; Gary Peltz |
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Publication Detail:
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Type: Journal Article Date: 2010-10-01 |
Journal Detail:
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Title: Anesthesia and analgesia Volume: 111 ISSN: 1526-7598 ISO Abbreviation: Anesth. Analg. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-25 Completed Date: 2011-01-07 Revised Date: 2011-03-29 |
Medline Journal Info:
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Nlm Unique ID: 1310650 Medline TA: Anesth Analg Country: United States |
Other Details:
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Languages: eng Pagination: 1534-42 Citation Subset: AIM; IM |
Affiliation:
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Department of Anesthesia, Stanford University, Stanford, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analgesics
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pharmacology Animals Anti-Inflammatory Agents / pharmacology Cells, Cultured Cesarean Section Chemokine CCL3 / metabolism Chemokine CXCL1 / metabolism Chemokines / metabolism* Disease Models, Animal Female Humans Inflammation Mediators / metabolism* Interleukin 1 Receptor Antagonist Protein / pharmacology Interleukin-1 / metabolism* Interleukin-1alpha / metabolism Interleukin-1beta / metabolism Interleukin-8 / metabolism Keratinocytes / drug effects, immunology*, radiation effects Male Mice Mice, Inbred C57BL Pain, Postoperative / immunology, prevention & control Pregnancy Randomized Controlled Trials as Topic Skin / drug effects, immunology, radiation effects, surgery* Sunburn / immunology Time Factors Translational Research Wound Healing* / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM079126-04/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Analgesics; 0/Anti-Inflammatory Agents; 0/CXCL1 protein, human; 0/Ccl3 protein, mouse; 0/Chemokine CCL3; 0/Chemokine CXCL1; 0/Chemokines; 0/Inflammation Mediators; 0/Interleukin 1 Receptor Antagonist Protein; 0/Interleukin-1; 0/Interleukin-1alpha; 0/Interleukin-1beta; 0/Interleukin-8; 147037-79-4/keratinocyte-derived chemokines |
| Comments/Corrections | |
Comment In:
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Anesth Analg. 2010 Dec;111(6):1335-6
[PMID:
21106965
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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