| Role of immune serum in the killing of Helicobacter pylori by macrophages. | |
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MedLine Citation:
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PMID: 20557358 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Helicobacter pylori infection can lead to the development of gastritis, peptic ulcers and gastric cancer, which makes this bacterium an important concern for human health. Despite evoking a strong immune response in the host, H. pylori persists, requiring complex antibiotic therapy for eradication. Here we have studied the impact of a patient's immune serum on H. pylori in relation to macrophage uptake, phagosome maturation, and bacterial killing. MATERIALS AND METHODS: Primary human macrophages were infected in vitro with both immune serum-treated and control H. pylori. The ability of primary human macrophages to kill H. pylori was characterized at various time points after infection. H. pylori phagosome maturation was analyzed by confocal immune fluorescence microscopy using markers specific for H. pylori, early endosomes (EEA1), late endosomes (CD63) and lysosomes (LAMP-1). RESULTS: Immune serum enhanced H. pylori uptake into macrophages when compared to control bacteria. However, a sufficient inoculum remained for recovery of viable H. pylori from macrophages, at 8 hours after infection, for both the serum-treated and control groups. Both serum-treated and control H. pylori phagosomes acquired EEA1 (15 minutes), CD63 and LAMP-1 (30 minutes). These markers were then retained for the rest of an 8 hour time course. CONCLUSIONS: While immune sera appeared to have a slight positive effect on bacterial uptake, both serum-treated and control H. pylori were not eliminated by macrophages. Furthermore, the same disruptions to phagosome maturation were observed for both serum-treated and control H. pylori. We conclude that to eliminate H. pylori, a strategy is required to restore the normal process of phagosome maturation and enable effective macrophage killing of H. pylori, following a host immune response. |
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Authors:
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Stacey Keep; Glenn Borlace; Ross Butler; Doug Brooks |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Helicobacter Volume: 15 ISSN: 1523-5378 ISO Abbreviation: Helicobacter Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-18 Completed Date: 2010-09-20 Revised Date: 2010-10-20 |
Medline Journal Info:
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Nlm Unique ID: 9605411 Medline TA: Helicobacter Country: United States |
Other Details:
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Languages: eng Pagination: 177-83 Citation Subset: IM |
Affiliation:
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University of South Australia, Adelaide, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD / analysis Cells, Cultured Colony Count, Microbial Endosomes / chemistry, microbiology Helicobacter pylori / immunology*, pathogenicity* Humans Immune Sera / immunology* Lysosome-Associated Membrane Glycoproteins / analysis Lysosomes / chemistry, microbiology Macrophages / immunology* Microbial Viability* Microscopy, Confocal Microscopy, Fluorescence Phagosomes / chemistry, microbiology Platelet Membrane Glycoproteins / analysis Time Factors Vesicular Transport Proteins / analysis |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/CD63 antigen; 0/Immune Sera; 0/LAMP1 protein, human; 0/Lysosome-Associated Membrane Glycoproteins; 0/Platelet Membrane Glycoproteins; 0/Vesicular Transport Proteins; 0/early endosome antigen 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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