Document Detail

Role of IL-1beta on the glutamine synthetase in retinal Müller cells under high glucose conditions.
MedLine Citation:
PMID:  19839866     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate (1) the role of cytokine interleukin-1beta on the glutamine synthetase in retinal Müller cells under high glucose condition, (2) the mechanism for down-regulation of glutamine synthetase in retinal Müller cells induced by interleukin-1beta under high glucose conditions. MATERIALS AND METHODS: The effect of interleukin-1beta on the expression of glutamine synthetase and c-Jun in retinal Müller cells under normal and high glucose conditions was measured by immunocytochemistry, Western blot, and real-time (RT) PCR, and was further confirmed by c-Jun siRNA method. RESULTS: Under high glucose conditions, interleukin-1beta significantly increased expression of c-Jun and decreased the expression of glutamine synthetase. When c-Jun gene was silenced by siRNA, interleukin-1beta could not decrease glutamine synthetase expression in high glucose concentrations. CONCLUSIONS: Interleukin-1beta decreases expression of glutamine synthetase via activation of c-Jun. The data suggested that interleukin-1beta may play a role in the development of diabetic retinopathy.
Xi Shen; Gezhi Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current eye research     Volume:  34     ISSN:  1460-2202     ISO Abbreviation:  Curr. Eye Res.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-10-20     Completed Date:  2009-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8104312     Medline TA:  Curr Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  727-36     Citation Subset:  IM    
Department of Ophthalmology, Ruijin Hospital, School of Medicine, The Jiaotong University, Shanghai, China.
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MeSH Terms
Animals, Newborn
Blotting, Western
Cells, Cultured
Fluorescent Antibody Technique, Indirect
Gene Silencing
Glucose / pharmacology*
Glutamate-Ammonia Ligase / genetics,  metabolism*
Interleukin-1beta / physiology*
Neuroglia / drug effects*,  metabolism
Proto-Oncogene Proteins c-jun / genetics,  metabolism
RNA, Messenger / metabolism
RNA, Small Interfering / physiology
Retinal Neurons / drug effects*,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Interleukin-1beta; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/RNA, Small Interfering; 50-99-7/Glucose; EC Ligase

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