| Role of the Ha-ras gene in the malignant transformation of rat liver oval cells. | |
| | |
MedLine Citation:
|
PMID: 9178826 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have shown that the oval cell line OC/CDE 22 can be transformed by the highly carcinogenic fjord-region diol epoxides of benzo[c]phenanthrene. Mutational activation of the ras proto-oncogene family has been proposed to be a critical event in the formation of tumors induced by polycyclic aromatic hydrocarbons. Therefore, we investigated whether in the earlier transformed OC/CDE 22 cells any point mutations were detected in the ras proto-oncogene. The results indicate that the malignant transformation of OC/CDE 22 cells by the 4 stereoisomeric benzo[c]phenanthrene diol epoxides in vitro is independent of activation of the Ha-ras proto-oncogene. In addition, Northern and Western blot analyses revealed no overexpression of the Ha-ras protooncogene in the transformed OC/CDE 22 cell lines. However, transfection of the OC/CDE 22 cells with an activated Ha-ras oncogene malignantly transformed the OC/CDE 22 cells, and the transfected cells served as precursor cells of tumors with a cholangiocellular morphology and phenotype. Our latter finding reinforces the view that OC/CDE 22 cells are committed to the bile duct epithelial cell lineage. |
| | |
Authors:
|
P Steinberg; H Frank; M Odenthal; H P Dienes; A Seidel |
Related Documents
:
|
2172246 - Modulation of lysosomal-associated membrane glycoproteins during retinoic acid-induced ... 3510726 - Malignant transformation of a preneoplastic hamster epidermal cell line by the ej c-ha-... 8076376 - Identification of egf as an angiogenic factor present in conditioned medium from human ... 17848576 - Juxtacrine activation of epidermal growth factor (egf) receptor by membrane-anchored he... 8856256 - Escherichia coli-macrophage interactions modulate mesangial cell proliferation and matr... 365746 - Mannose binding and epithelial cell adherence of escherichia coli. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: International journal of cancer. Journal international du cancer Volume: 71 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 1997 May |
Date Detail:
|
Created Date: 1997-06-27 Completed Date: 1997-06-27 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 680-5 Citation Subset: IM |
Affiliation:
|
Institute of Toxicology, University of Mainz, Germany. steinber@mzdmza.zdv.uni-mainz.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bile Ducts / cytology Carcinogens Carcinoma / genetics Cell Differentiation Cell Line / drug effects Cell Lineage Cell Transformation, Neoplastic / chemically induced, genetics* Epithelial Cells Gene Expression Regulation, Neoplastic Genes, ras* Humans Liver / cytology* Liver Neoplasms, Experimental / chemically induced, genetics* Neoplasm Transplantation Neoplastic Stem Cells / metabolism, ultrastructure Phenanthrenes Rats Rats, Sprague-Dawley Transfection Urinary Bladder Neoplasms / genetics |
| Chemical | |
Reg. No./Substance:
|
0/Carcinogens; 0/Phenanthrenes; 111001-48-0/1,2-epoxy-3,4-dihydroxy-1,2,3,4-tetrahydrobenzo(c)phenanthrene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transduction of cytosine deaminase gene makes rat glioma cells highly sensitive to 5-fluorocytosine.
Next Document: Correlation of repressed transcription of alpha-tocopherol transfer protein with serum alpha-tocophe...