Document Detail


Role of the Ha-ras gene in the malignant transformation of rat liver oval cells.
MedLine Citation:
PMID:  9178826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have shown that the oval cell line OC/CDE 22 can be transformed by the highly carcinogenic fjord-region diol epoxides of benzo[c]phenanthrene. Mutational activation of the ras proto-oncogene family has been proposed to be a critical event in the formation of tumors induced by polycyclic aromatic hydrocarbons. Therefore, we investigated whether in the earlier transformed OC/CDE 22 cells any point mutations were detected in the ras proto-oncogene. The results indicate that the malignant transformation of OC/CDE 22 cells by the 4 stereoisomeric benzo[c]phenanthrene diol epoxides in vitro is independent of activation of the Ha-ras proto-oncogene. In addition, Northern and Western blot analyses revealed no overexpression of the Ha-ras protooncogene in the transformed OC/CDE 22 cell lines. However, transfection of the OC/CDE 22 cells with an activated Ha-ras oncogene malignantly transformed the OC/CDE 22 cells, and the transfected cells served as precursor cells of tumors with a cholangiocellular morphology and phenotype. Our latter finding reinforces the view that OC/CDE 22 cells are committed to the bile duct epithelial cell lineage.
Authors:
P Steinberg; H Frank; M Odenthal; H P Dienes; A Seidel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  71     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-06-27     Completed Date:  1997-06-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  680-5     Citation Subset:  IM    
Affiliation:
Institute of Toxicology, University of Mainz, Germany. steinber@mzdmza.zdv.uni-mainz.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Ducts / cytology
Carcinogens
Carcinoma / genetics
Cell Differentiation
Cell Line / drug effects
Cell Lineage
Cell Transformation, Neoplastic / chemically induced,  genetics*
Epithelial Cells
Gene Expression Regulation, Neoplastic
Genes, ras*
Humans
Liver / cytology*
Liver Neoplasms, Experimental / chemically induced,  genetics*
Neoplasm Transplantation
Neoplastic Stem Cells / metabolism,  ultrastructure
Phenanthrenes
Rats
Rats, Sprague-Dawley
Transfection
Urinary Bladder Neoplasms / genetics
Chemical
Reg. No./Substance:
0/Carcinogens; 0/Phenanthrenes; 111001-48-0/1,2-epoxy-3,4-dihydroxy-1,2,3,4-tetrahydrobenzo(c)phenanthrene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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