Document Detail


The role of GH in adipose tissue: lessons from adipose-specific GH receptor gene-disrupted mice.
MedLine Citation:
PMID:  23349524     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
GH receptor (GHR) gene-disrupted mice (GHR-/-) have provided countless discoveries as to the numerous actions of GH. Many of these discoveries highlight the importance of GH in adipose tissue. For example GHR-/- mice are insulin sensitive yet obese with preferential enlargement of the sc adipose depot. GHR-/- mice also have elevated levels of leptin, resistin, and adiponectin, compared with controls leading some to suggest that GH may negatively regulate certain adipokines. To help clarify the role that GH exerts specifically on adipose tissue in vivo, we selectively disrupted GHR in adipose tissue to produce Fat GHR Knockout (FaGHRKO) mice. Surprisingly, FaGHRKOs shared only a few characteristics with global GHR-/- mice. Like the GHR-/- mice, FaGHRKO mice are obese with increased total body fat and increased adipocyte size. However, FaGHRKO mice have increases in all adipose depots with no improvements in measures of glucose homeostasis. Furthermore, resistin and adiponectin levels in FaGHRKO mice are similar to controls (or slightly decreased) unlike the increased levels found in GHR-/- mice, suggesting that GH does not regulate these adipokines directly in adipose tissue in vivo. Other features of FaGHRKO mice include decreased levels of adipsin, a near-normal GH/IGF-1 axis, and minimal changes to a large assortment of circulating factors that were measured such as IGF-binding proteins. In conclusion, specific removal of GHR in adipose tissue is sufficient to increase adipose tissue and decrease circulating adipsin. However, removal of GHR in adipose tissue alone is not sufficient to increase levels of resistin or adiponectin and does not alter glucose metabolism.
Authors:
Edward O List; Darlene E Berryman; Kevin Funk; Elahu S Gosney; Adam Jara; Bruce Kelder; Xinyue Wang; Laura Kutz; Katie Troike; Nicholas Lozier; Vincent Mikula; Ellen R Lubbers; Han Zhang; Clare Vesel; Riia K Junnila; Stuart J Frank; Michal M Masternak; Andrzej Bartke; John J Kopchick
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-24
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  27     ISSN:  1944-9917     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-26     Completed Date:  2013-10-17     Revised Date:  2014-03-06    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  524-35     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology,  metabolism
Adipokines / blood
Adipose Tissue / metabolism*
Adiposity
Animals
Body Composition
Body Weight
Cell Count
Cell Size
Cytokines / blood
Female
Gene Deletion*
Glucose / metabolism
Growth Hormone / metabolism*
Homeostasis
Insulin-Like Growth Factor Binding Proteins / metabolism
Insulin-Like Growth Factor I / metabolism
Liver / metabolism
Male
Mice
Mice, Knockout
Organ Size
Organ Specificity
Receptors, Somatotropin / metabolism*
Triglycerides / metabolism
Grant Support
ID/Acronym/Agency:
AG032290/AG/NIA NIH HHS; DK083729/DK/NIDDK NIH HHS; DK58259/DK/NIDDK NIH HHS; P01AG031736/AG/NIA NIH HHS; R01 AG032290/AG/NIA NIH HHS; R01 DK058259/DK/NIDDK NIH HHS; U01HG004080/HG/NHGRI NIH HHS; U01HG004085/HG/NHGRI NIH HHS; U42RR024244/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Adipokines; 0/Cytokines; 0/Insulin-Like Growth Factor Binding Proteins; 0/Receptors, Somatotropin; 0/Triglycerides; 67763-96-6/Insulin-Like Growth Factor I; 9002-72-6/Growth Hormone; IY9XDZ35W2/Glucose
Comments/Corrections

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