| Role of Fyn kinase in oocyte developmental potential. | |
| | |
MedLine Citation:
|
PMID: 20591331 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fyn kinase is highly expressed in oocytes, with inhibitor and dominant-negative studies suggesting a role in the signal transduction events during egg activation. The purpose of the present investigation was to test the hypothesis that Fyn is required for calcium signalling, meiosis resumption and pronuclear congression using the Fyn-knockout mouse as a model. Accelerated breeding studies revealed that Fyn-null females produced smaller litter sizes at longer intervals and exhibited a rapid decline in pup production with increasing age. Fyn-null females produced a similar number of oocytes, but the frequency of immature oocytes and mature oocytes with spindle chromosome abnormalities was significantly higher than in controls. Fertilised Fyn-null oocytes frequently (24%) failed to undergo pronuclear congression and remained at the one-cell stage. Stimulation with gonadotropins increased the number of oocytes ovulated, but did not overcome the above defects. Fyn-null oocytes overexpressed Yes kinase in an apparent effort to compensate for the loss of Fyn, yet still exhibited an altered pattern of protein tyrosine phosphorylation. In summary, Fyn-null female mice exhibit reduced fertility that appears to result from actin cytoskeletal defects rather than calcium signalling. These defects cause developmental arrest during oocyte maturation and pronuclear congression. |
| | |
Authors:
|
Jinping Luo; Lynda K McGinnis; William H Kinsey |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Reproduction, fertility, and development Volume: 22 ISSN: 1031-3613 ISO Abbreviation: Reprod. Fertil. Dev. Publication Date: 2010 |
Date Detail:
|
Created Date: 2010-07-01 Completed Date: 2010-09-14 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8907465 Medline TA: Reprod Fertil Dev Country: Australia |
Other Details:
|
Languages: eng Pagination: 966-76 Citation Subset: IM |
Affiliation:
|
Center for Reproductive Sciences, University of Kansas Medical Center, Kansas City, KS 66160, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Calcium Signaling / physiology* Cell Nucleus / genetics, metabolism Cytoskeleton / genetics, metabolism* Female Litter Size / physiology Meiosis / physiology* Mice Mice, Knockout Microscopy, Confocal Oocytes / metabolism* Ovulation / genetics, metabolism Ovulation Induction Proto-Oncogene Proteins c-fyn / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
14846//PHS HHS; P20RR024214/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
EC 2.7.10.2/Proto-Oncogene Proteins c-fyn |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A-type lamin dynamics in bovine somatic cell nuclear transfer embryos.
Next Document: Prolonged interval between fusion and activation impairs embryonic development by inducing chromosom...