| Role of Focal Adhesion Kinase in Lung Remodeling of Endotoxemic Rats. | |
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MedLine Citation:
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PMID: 22293597 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ABSTRACT: Despite significant advances in the care of critically ill patients, Acute Lung Injury (ALI) continues to be a complex problem with high mortality. The present study was designed to characterize early LPS-induced pulmonary injury and siRNA Focal Adhesion Kinase (siFAK) as a possible therapeutic tool in the septic lung remodeling process. Male Wistar rats were assigned into: endotoxemic group and control group. Total collagen deposition was performed 8, 16 and 24 hours after LPS injection. FAK expression, interstitial and vascular collagen deposition, and pulmonary mechanics were analyzed at 24 hour. Intravenous injection of small interfering RNA targeting focal adhesion kinase was employed in order to silence expression of the kinase in pulmonary tissue. FAK, total collagen deposition and pulmonary mechanics showed increased in LPS group. Types I, III and V collagen showed increase in pulmonary parenchyma but only type V increased in vessels 24 h after LPS injection. FAK silencing prevented lung remodeling in pulmonary parenchyma at 24 hour. In conclusion, LPS induced a precocious and important lung remodeling. There was fibrotic response in lung characterized by increased amount in total and specific type collagen. These data may explain the frequent clinical presentation during sepsis of reduced lung compliance, oxygen diffusion and pulmonary hypertension. The fact that FAK silencing was protective against lung collagen deposition underscores the therapeutic potential of FAK targeting by siRNA. |
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Authors:
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Ricardo Costa Petroni; Walcy R Teodoro; Maria Carolina Guido; Hermes Vieira Barbeiro; Fétima Abatepaulo; Mariana Theobaldo; Paolo Cesare Biselli |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-27 |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: - ISSN: 1540-0514 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-2-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Emergency Medicine Department, Medical School, University of São Paulo, São Paulo, Brazil; 2Rheumatology Division of Internal Medicine Department - Medical School of University of Sao Paulo, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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