| Role of ferredoxin and flavodoxins in Bacillus subtilis fatty acid desaturation. | |
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MedLine Citation:
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PMID: 21665975 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Bacillus subtilis acyl lipid desaturase (Δ5-Des) is an iron-dependent integral membrane protein able to selectively introduce double bonds into long-chain fatty acids. In the last decade since its discovery, the molecular mechanism of Δ5-Des expression has been studied extensively. However, the mechanism of desaturation, which must rely on unknown bacterial proteins for electron transfer, has not yet been explored. The B. subtilis genome encodes three proteins that can act as potential electron donors of Δ5-Des, ferredoxin (Fer) and two flavodoxins (Flds) (YkuN and YkuP), which are encoded by the ykuNOP operon. Here we report that the disruption of either the fer gene or the ykuNOP operon decreases the desaturation of palmitic acid by ∼30%. Nevertheless, a fer ykuNOP mutant abolished the desaturation reaction almost completely. Our results establish Fer and the two Flds as redox partners for Δ5-Des and suggest that the Fer and Fld proteins could function physiologically in the biosynthesis of unsaturated fatty acids in B. subtilis. Although Flds have extensively been described as partners in a number of redox processes, this is the first report describing their role as electron donors in the fatty acid desaturation reaction. |
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Authors:
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Lorena Chazarreta-Cifre; Leticia Martiarena; Diego de Mendoza; Silvia G Altabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-06-10 |
Journal Detail:
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Title: Journal of bacteriology Volume: 193 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-29 Completed Date: 2011-09-27 Revised Date: 2012-02-01 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 4043-8 Citation Subset: IM |
Affiliation:
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Instituto de Biología Molecular y Celular de Rosario, and Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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metabolism* Bacterial Proteins / genetics, metabolism Fatty Acid Desaturases / metabolism* Fatty Acids / metabolism* Ferredoxins / metabolism* Flavodoxin / metabolism* Gene Expression Regulation, Bacterial / physiology Mutation |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Fatty Acids; 0/Ferredoxins; 0/Flavodoxin; EC 1.14.19.-/Fatty Acid Desaturases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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