Document Detail

Role of nociceptin/orphanin FQ and the pseudopeptide [Phe1Psi(CH2NH)Gly2]-nociceptin(1-13)-NH2 and their interaction with classic opioids in the modulation of thermonociception in the land snail Helix aspersa.
MedLine Citation:
PMID:  18096155     Owner:  NLM     Status:  MEDLINE    
The role in nociception of nociceptin/orphanin FQ (N/OFQ) and its receptor, the opioid receptor-like 1 (NOP), remains unclear because this peptide has been implicated in both suppression and enhancement of nociception. The present work characterises the effects of N/OFQ and the NOP receptor antagonist, the pseudopeptide [Phe(1)Psi(CH(2)NH)Gly(2)]-nociceptin(1-13)-NH(2) (Phe(1)Psi), on thermonociception in the snail Helix aspersa using the hot plate assay. Additionally, the possible interaction of each of these compounds with morphine or dynorphin A(1-17) and naloxone was studied. Compounds were administered into the hemocoel cavity of H. aspersa and the latency to the aversive withdrawal behaviour recorded. Dose-response and time course curves were done. N/OFQ and naloxone produced a similar dose-dependent pronociceptive effect; however, N/OFQ reached its peak effect earlier and was 30 times more potent than naloxone. [Phe(1)Psi(CH(2)NH)Gly(2)]-nociceptin(1-13)-NH(2) and the opioid agonists, morphine and dynorphin A(1-17) produced antinociception with a similar efficacy, but [Phe(1)Psi(CH(2)NH)Gly(2)]-nociceptin(1-13)-NH(2) reached its peak effect more rapidly and lasted longer than that of dynorphin A(1-17) and morphine. [Phe(1)Psi(CH(2)NH)Gly(2)]-nociceptin(1-13)-NH(2) was 50 times less potent than dynorphin A(1-17), but 30 times more potent than morphine. N/OFQ significantly reduced morphine and dynorphin A(1-17)-induced antinociception. Combined administration of low doses of [Phe(1)Psi(CH(2)NH)Gly(2)]-nociceptin(1-13)-NH(2) and morphine or dynorphin A(1-17) produced a potent antinociceptive effect. Sub-effective doses of naloxone and N/OFQ also synergised to produce pronociception. Data suggest that these two opioid classes regulate nociception through parallel systems. The H. aspersa model appears as a valuable experimental preparation to continue the study of these opioid receptor systems.
Carolina Miller-Pérez; Eduardo Sánchez-Islas; Francisco Pellicer; Gabriela Rodríguez-Manzo; Silvia L Cruz; Martha León-Olea
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-28
Journal Detail:
Title:  European journal of pharmacology     Volume:  581     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-04     Completed Date:  2008-05-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  77-85     Citation Subset:  IM    
Laboratorio de Histología y Microscopía Electrónica, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría, Calzada México-Xochimilco 101, Col. San Lorenzo Huipulco, Tlalpan. México D.F., C.P. 14370, México.
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MeSH Terms
Dose-Response Relationship, Drug
Drug Interactions
Dynorphins / pharmacology
Helix (Snails) / physiology*
Hot Temperature
Morphine / pharmacology
Motor Activity / drug effects
Naloxone / pharmacology
Opioid Peptides / pharmacology*
Pain / physiopathology*
Peptide Fragments / pharmacology*
Reaction Time / drug effects
Receptors, Opioid / antagonists & inhibitors*
Reg. No./Substance:
0/Opioid Peptides; 0/Peptide Fragments; 0/Receptors, Opioid; 0/nociceptin; 0/nociceptin (1-13)-NH2, Phe(1)-psi(CH2-NH)-Gly(2)-; 0/nociceptin receptor; 465-65-6/Naloxone; 57-27-2/Morphine; 74913-18-1/Dynorphins

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