| Role of electrolytes and glucose in the insulin-induced electrochemical effect in sheep pleura. | |
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MedLine Citation:
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PMID: 20162507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Insulin induces electrochemical alterations in sheep visceral and parietal pleura, an effect abolished by the Na (+)-channel blocker amiloride and the Na (+)-K (+) pump inhibitor ouabain. The aim of this study was to further investigate the role of different electrolytes and glucose in these electrochemical changes. MATERIALS AND METHODS: Sheep pleural specimens were mounted in Ussing chambers. Insulin (10 (-7)M) was added mesothelially in Na (+), K (+), Ca (2+)-free, low H (+) and glucose solutions. In other experiments, specimens were pretreated with K (+) and Ca (2+)-free Krebs solutions. Trans-mesothelial Resistance was determined. RESULTS: Insulin did not increase Trans-mesothelial Resistance of visceral and parietal pleura in K (+)-free (p=0.008 and p=0.028 respectively), Ca (2+)-free (p=0.006 and p=0.012 respectively) and low glucose (p=0.009 and p=0.03 respectively) solutions. This effect was totally inhibited in Na (+)-free solutions or in specimens pretreated with Ca (2+) -free Krebs solution and partially inhibited, when low H (+) solutions were used (p=0.042 for visceral and p=0.045 for parietal). CONCLUSION: Insulin-induced electrochemical changes in sheep pleura are mainly associated with alterations in Na (+) and Ca (2+) concentrations. Since amiloride and ouabain abolish these electrochemical changes, it may be suggested that insulin could influence the pleural fluid recycling, mainly via the Na (+) transportation system, irrespective of the glucose content. |
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Authors:
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V K Kouritas; C H Hatzoglou; K I Gourgoulianis; P A Molyvdas |
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Publication Detail:
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Type: Journal Article Date: 2010-02-16 |
Journal Detail:
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Title: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association Volume: 118 ISSN: 1439-3646 ISO Abbreviation: Exp. Clin. Endocrinol. Diabetes Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-10 Completed Date: 2010-08-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9505926 Medline TA: Exp Clin Endocrinol Diabetes Country: Germany |
Other Details:
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Languages: eng Pagination: 328-32 Citation Subset: IM |
Affiliation:
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Department of Physiology, Medical School of Larissa, University of Thessaly, Greece. kouritas@otenet.gr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / pharmacology Electrolytes / metabolism* Electrophysiology / methods Epithelium / drug effects, physiology Female Glucose / metabolism* Insulin / pharmacology* Male Pleura / drug effects, physiology* Potassium / pharmacology Sheep Sheep, Domestic |
| Chemical | |
Reg. No./Substance:
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0/Electrolytes; 11061-68-0/Insulin; 50-99-7/Glucose; 7440-09-7/Potassium; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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