| The role of dark carbon dioxide fixation in root nodules of soybean. | |
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MedLine Citation:
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PMID: 16664774 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The magnitude and role of dark CO(2) fixation were examined in nodules of intact soybean plants (Harosoy 63 x Rhizobium japonicum strain USDA 16). The estimated rate of nodule dark CO(2) fixation, based on a 2 minute pulse-feed with (14)CO(2) under saturating conditions, was 102 micromoles per gram dry weight per hour. This was equivalent to 14% of net nodule respiration. Only 18% of this CO(2) fixation was estimated to be required for organic and amino acid synthesis for growth and export processes. The major portion (75-92%) of fixed label was released as CO(2) within 60 minutes. The labeling pattern during pulse-chase experiments was consistent with CO(2) fixation by phosphoenolpyruvate carboxylase. During the chase, the greatest loss of label occurred in organic acids. Exposure of nodulated roots to Ar:O(2) (80:20) did not affect dark CO(2) fixation, while exposure to O(2):CO(2) (95:5) resulted in 54% inhibition. From these results, it was concluded that at least 66% of dark CO(2) fixation in soybean may be involved with the production of organic acids, which when oxidized would be capable of providing at least 48% of the requirement for ATP equivalents to support nitrogenase activity. |
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Authors:
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B J King; D B Layzell; D T Canvin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant physiology Volume: 81 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1986 May |
Date Detail:
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Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-14 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 200-5 Citation Subset: - |
Affiliation:
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Department of Biology, Queen's University, Kingston, Ontario K7L 3N6 Canada. |
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