| The role of cytochrome c on apoptosis induced by Anagrapha falcifera multiple nuclear polyhedrosis virus in insect Spodoptera litura cells. | |
| | |
MedLine Citation:
|
PMID: 22952575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
There are conflicting reports on the role of cytochrome c during insect apoptosis. Our previous studies have showed that cytochrome c released from the mitochondria was an early event by western blot analysis and caspase-3 activation was closely related to cytochrome c release during apoptosis induced by baculovirus in Spodoptera litura cells (Sl-1 cell line). In the present study, alteration in mitochondrial morphology was observed by transmission electron microscopy, and cytochrome c release from mitochondria in apoptotic Sl-1 cells induced with Anagrapha falcifera multiple nuclear polyhedrosis virus (AfMNPV) has further been confirmed by immunofluoresence staining protocol, suggesting that structural disruption of mitochondria and the release of cytochrome c are important events during Lepidoptera insect cell apoptosis. We also used Sl-1 cell-free extract system and the technique of RNA interference to further investigate the role of cytochrome c in apoptotic Sl-1 cells induced by AfMNPV. Caspase-3 activity in cell-free extracts supplemented with exogenous cytochrome c was determined and showed an increase with the extension of incubation time. DsRNA-mediated silencing of cytochrome c resulted in the inhibition of apoptosis and protected the cells from AfMNPV-induced cell death. Silencing of expression of cytochrome c had a remarkable effect on pro-caspase-3 and pro-caspase-9 activation and resulted in the reduction of caspase-3 and caspase-9 activity in Sl-1 cells undergoing apoptosis. Caspase-9 inhibitor could inhibit activation of pro-caspase-3, and the inhibition of the function of Apaf-1 with FSBA blocked apoptosis, hinting that Apaf-1 could be involved in Sl-1 cell apoptosis induced by AfMNPV. Taken together, these results strongly demonstrate that cytochrome c plays an important role in apoptotic signaling pathways in Lepidopteran insect cells. |
| | |
Authors:
|
Kaiyu Liu; Duanyang Shu; Na Song; Zhongchao Gai; Yuan Yuan; Juan Li; Min Li; Shuying Guo; Jianxin Peng; Huazhu Hong |
Related Documents
:
|
22920925 - Bodipy-labeled dc-sign-targeting glycodendrons efficiently internalize and route to lys... 23624075 - An emerging role for serine protease inhibitors in t lymphocyte immunity and beyond. 9568105 - Tempicol-2 (4-hydroxy-4-(2-picolyl)-2,2,6,6-tetramethylpiperidine-1-oxyl), a stable fre... 23363775 - Activation of microglial cells triggers a release of brain-derived neurotrophic factor ... 9708185 - Down-regulation of cd28 via fas (cd95): influence of cd28 on t-cell apoptosis. 19706755 - Dual therapeutic efficacy of vinblastine as a unique chemotherapeutic agent capable of ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-08-28 |
Journal Detail:
|
Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
|
Created Date: 2012-09-06 Completed Date: 2013-02-19 Revised Date: 2013-04-02 |
Medline Journal Info:
|
Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
|
Languages: eng Pagination: e40877 Citation Subset: IM |
Affiliation:
|
Institute of Entomology, Central China Normal University, Wuhan, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis* Apoptotic Protease-Activating Factor 1 / metabolism Caspase 3 / metabolism Caspase 9 / metabolism Cell-Free System Cytochromes c / metabolism* Dactinomycin / pharmacology Flow Cytometry / methods Gene Silencing Insects Microscopy, Fluorescence / methods Mitochondria / metabolism Nucleopolyhedrovirus / metabolism* RNA Interference RNA, Double-Stranded / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Apoptotic Protease-Activating Factor 1; 0/RNA, Double-Stranded; 50-76-0/Dactinomycin; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Supporting adherence to antiretroviral therapy with mobile phone reminders: results from a cohort in...
Next Document: Cancer association study of aminoacyl-tRNA synthetase signaling network in glioblastoma.