| The Role of Cholesterol In Ultraviolet Light B-Induced Apoptosis(†). | |
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MedLine Citation:
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PMID: 22077874 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Modification of major lipid raft components, such as cholesterol and ceramide, plays a role in regulation of programmed cell death under various stimuli. However, the relationship between cholesterol level modification and the activation of apoptotic signaling cascades upon ultraviolet B light has not been established. In this report, we demonstrate that upon UVB irradiation cholesterol levels in membrane rafts of skin cells increase, which leads to Fas aggregation in the rafts. Utilizing a continuous velocity floatation technique, we show that Fas accumulated in the lipid rafts of human melanoma M624 cells after UVB-irradiation. The subsequent events of DISC formation were also detected in the lipid raft fractions. Depletion of cholesterol by methyl-β-cyclodextrin reduces Fas aggregation, while overloading increases. Disruption of lipid rafts also prevents Daxx from dissociating from Fas in the lipid rafts, which is accompanied with a reduced apoptotic, but increased non-apoptotic death of UVB-irradiated human keratinocytes, HaCaT cells. Results indicate that cholesterol located in the plasma membrane of skin cells is required for lipid raft domain formation and activation of UVB-induced apoptosis. |
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Authors:
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Kimberly S George; Walid Elyassaki; Qiong Wu; Shiyong Wu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-12 |
Journal Detail:
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Title: Photochemistry and photobiology Volume: - ISSN: 1751-1097 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Photochemistry and Photobiology © 2011 American Society for Photobiology. |
Affiliation:
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Edison Biotechnology Institute and Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio 45701, USA Department of Chemistry, Marietta College, Marietta, OH 45750, USA Battelle Memorial Institute, Charlottesville, VA 22902, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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