Document Detail


Role of β-catenin in post-meiotic male germ cell differentiation.
MedLine Citation:
PMID:  22125654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Though roles of β-catenin signaling during testis development have been well established, relatively little is known about its role in postnatal testicular physiology. Even less is known about its role in post-meiotic germ cell development and differentiation. Here, we report that β-catenin is highly expressed in post-meiotic germ cells and plays an important role during spermiogenesis in mice. Spermatid-specific deletion of β-catenin resulted in significantly reduced sperm count, increased germ cell apoptosis and impaired fertility. In addition, ultrastructural studies show that the loss of β-catenin in post-meiotic germ cells led to acrosomal defects, anomalous release of immature spermatids and disruption of adherens junctions between Sertoli cells and elongating spermatids (apical ectoplasmic specialization; ES). These defects are likely due to altered expression of several genes reportedly involved in Sertoli cell-germ cell adhesion and germ cell differentiation, as revealed by gene expression analysis. Taken together, our results suggest that β-catenin is an important molecular link that integrates Sertoli cell-germ cell adhesion with the signaling events essential for post-meiotic germ cell development and maturation. Since β-catenin is also highly expressed in the Sertoli cells, we propose that binding of germ cell β-catenin complex to β-catenin complex on Sertoli cell at the apical ES surface triggers a signaling cascade that regulates post-meiotic germ cell differentiation.
Authors:
Yao-Fu Chang; Jennifer S Lee-Chang; Krystle Y Harris; Amiya P Sinha-Hikim; Manjeet K Rao
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-11-18
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-11-29     Completed Date:  2012-04-02     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e28039     Citation Subset:  IM    
Affiliation:
Greehey Children's Cancer Research Institute, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE30773
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / genetics,  physiology
Cell Adhesion / genetics,  physiology
Cell Differentiation / genetics,  physiology*
Cell Polarity / genetics,  physiology
Female
Gene Expression Profiling
Immunohistochemistry
Male
Meiosis / genetics
Mice
Mice, Knockout
Microscopy, Electron
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Sertoli Cells / cytology,  metabolism
Spermatogenesis / genetics,  physiology*
Spermatozoa / cytology*,  metabolism,  ultrastructure
Time Factors
beta Catenin / genetics,  metabolism,  physiology*
Grant Support
ID/Acronym/Agency:
HD057118/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/beta Catenin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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