Document Detail


Role of calcium independent phospholipase a(2) in maintaining mitochondrial membrane potential and preventing excessive exocytosis in PC12 cells.
MedLine Citation:
PMID:  21116712     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This study was carried out to elucidate the effects of calcium independent phospholipase A(2) (iPLA(2)) on mitochondrial function and exocytosis in neuroendocrine cells. iPLA(2) mRNA and protein were detected in cell lysates and mitochondria from PC12 cells. Treatment of cells with the iPLA(2) inhibitor, bromoenol lactone (BEL), resulted in reduction in the mitochondrial membrane potential. Increase in membrane capacitance and number of spikes at amperometry, indicating exocytosis, were detected from PC12 cells after treatment with BEL. The induced exocytosis was abolished by pre-incubation of cells with the antioxidant, glutathione monoethyl ester, spin-trap/free radical scavenger, PBN, or inhibitors of the mitochondrial permeability transition pore, cyclosporine A and bongkrekic acid. These findings indicate that inhibition of iPLA(2) results in excessive exocytosis through increased oxidative damage (or failure to repair such damage) and defects in mitochondrial function. A similar process may occur in neurons with mutations in iPLA(2), leading to neuronal injury.
Authors:
May-Thu Ma; Jin-Fei Yeo; Akhlaq A Farooqui; Wei-Yi Ong
Publication Detail:
Type:  Journal Article     Date:  2010-11-30
Journal Detail:
Title:  Neurochemical research     Volume:  36     ISSN:  1573-6903     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  347-54     Citation Subset:  IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, National University of Singapore, Singapore, 119260, Singapore.
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