Document Detail

Role of the CD45 (T-200) molecule in anti-CD3-triggered T cell-mediated cytotoxicity.
MedLine Citation:
PMID:  2972397     Owner:  NLM     Status:  MEDLINE    
NK-depleted human peripheral blood lymphocytes can be modulated with anti-CD3 to kill certain targets during 3-hr cytotoxicity assays. When triggered by anti-CD3 antibody, these effector T cells killed only NK-sensitive targets, such as K562 and HEL 92.1.7, and NK-resistant targets, such as Daudi, whose killing is inhibited by anti-CD45 (T-200) monoclonal antibodies, such as 13.3. NK-sensitive targets, MOLT-4, U266/AF10, Jurkat, and CCFR-CEM, and 10 NK-resistant cell lines, including Raji, IM-9, U698, U937, and GM-1056, whose killing is not inhibited by anti-CD45 monoclonal antibodies, were not killed by alpha-CD3-T effectors, suggesting that the CD45 molecule may be involved in the killing process. Anti-CD3-triggered T cell killing of target cells was inhibited greater than 95% by the monoclonal antibody 13.3. This inhibition of cytotoxicity by 13.3 was not due to competition of this IgG1 antibody for Fc receptor binding site on the target cell, since the IgG1 monoclonal antibody anti-beta 2-microglobulin did not block cytotoxicity. Single cell assays and calcium pulse assays showed that CD45 is involved in a postbinding, pre-calcium-dependent stage, similar to that shown for NK cytotoxicity. There was a relative shift of importance of different epitopes of CD45 in anti-CD3-T cytotoxicity compared to NK cytotoxicity. Anti-CD45 antibodies which bind to the C terminus end of the molecule played a more important role in anti-CD3-T cytotoxicity than NK cytotoxicity. Thus, a subset of T cells exists that exhibits anti-CD3-triggered non-MHC-restricted killing of certain NK-sensitive and NK-resistant targets in association with a CD45 molecule which is functionally different from the NK CD45 molecule.
R L Deem; F Shanahan; A Niederlehner; S R Targan
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular immunology     Volume:  117     ISSN:  0008-8749     ISO Abbreviation:  Cell. Immunol.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1988-12-14     Completed Date:  1988-12-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-110     Citation Subset:  IM    
Department of Medicine, UCLA School of Medicine 90024.
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MeSH Terms
Antibodies, Monoclonal / immunology
Antigen-Antibody Reactions
Antigens, CD3
Antigens, CD45
Antigens, Differentiation / physiology*
Antigens, Differentiation, T-Lymphocyte / physiology*
Cytotoxicity, Immunologic*
Histocompatibility Antigens / physiology*
Immunity, Cellular*
Immunity, Innate
Killer Cells, Natural / immunology
Receptors, Antigen, T-Cell / physiology*
Receptors, Fc / physiology
T-Lymphocytes / immunology*
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD3; 0/Antigens, Differentiation; 0/Antigens, Differentiation, T-Lymphocyte; 0/Histocompatibility Antigens; 0/Receptors, Antigen, T-Cell; 0/Receptors, Fc; EC, CD45

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