Document Detail


Role of CD44 and hyaluronan in neutrophil recruitment.
MedLine Citation:
PMID:  15585887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lymphocyte CD44 interactions with hyaluronan localized on the endothelium have been demonstrated to mediate rolling and regulate lymphocyte entry into sites of chronic inflammation. Because neutrophils also express CD44, we investigated the role of CD44 and hyaluronan in the multistep process of neutrophil recruitment. CD44(-/-) and wild-type control mice were intrascrotally injected with the neutrophil-activating chemokine, MIP-2, and leukocyte kinetics in the cremasteric microcirculation were investigated 4 h subsequently using intravital microscopy. Neither the rolling flux nor the rolling velocities were decreased in CD44(-/-) mice relative to wild-type mice. In vitro, neutrophils did not roll on the CD44 ligand hyaluronan, consistent with the in vivo data that CD44/hyaluronan did not mediate rolling. However, the number of adherent leukocytes in the venule was decreased by 65% in CD44(-/-) mice compared with wild-type mice. Leukocyte emigration was also greatly decreased in the CD44(-/-) mice. The same decrease in adhesion and emigration was observed in the wild-type mice given hyaluronidase. Histology revealed neutrophils as being the dominant infiltrating population. We generated chimeric mice that express CD44 either on their leukocytes or on their endothelium and found that CD44 on both the endothelium and neutrophils was important for optimal leukocyte recruitment into tissues. Of those neutrophils that emigrated in wild-type and CD44(-/-) mice, there was no impairment in migration through the interstitium. This study suggests that CD44 can mediate some neutrophil adhesion and emigration, but does not appear to affect subsequent migration within tissues.
Authors:
Adil I Khan; Steven M Kerfoot; Bryan Heit; Lixin Liu; Graciela Andonegui; Brian Ruffell; Pauline Johnson; Paul Kubes
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  173     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-08     Completed Date:  2005-02-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7594-601     Citation Subset:  AIM; IM    
Affiliation:
Immunology Research Group, Department of Physiology and Biophysics, University of Calgary Medical Center, Calgary, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD44 / biosynthesis,  genetics,  metabolism,  physiology*
Cell Adhesion / genetics,  immunology
Cell Movement / genetics,  immunology
Chemokine CXCL2
Chemokines / administration & dosage
Chemotaxis, Leukocyte / genetics,  immunology
Disease Models, Animal
Endothelium, Vascular / immunology,  metabolism,  pathology
Hyaluronic Acid / biosynthesis,  metabolism,  physiology*
Inflammation / genetics,  immunology,  pathology
Ligands
Mice
Mice, Inbred C57BL
Mice, Knockout
Muscle, Skeletal / blood supply,  immunology,  pathology
Neutrophil Infiltration / genetics,  immunology*
Neutrophils / immunology,  metabolism,  pathology
Radiation Chimera / immunology
Chemical
Reg. No./Substance:
0/Antigens, CD44; 0/Chemokine CXCL2; 0/Chemokines; 0/Cxcl2 protein, mouse; 0/Ligands; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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