| The Role of Basic Amino Acids in the Molecular Recognition of Hydroxyapatite by Statherin using Solid State NMR. | |
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MedLine Citation:
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PMID: 20676391 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Organisms use proteins such as statherin to control the growth of hydroxyapatite (HAP), which is the principal component of teeth and bone. Though much emphasis has been placed on the acidic character of these proteins, the role of their basic amino acids is not well understood. In this work, solid state nuclear magnetic resonance was used to probe the interaction of the basic arginine side chains with the HAP surface. Statherin samples were individually labeled at each arginine site, and the distance to the surface was measured using the Rotational Echo DOuble Resonance (REDOR) technique. The results indicate a strong coupling between the R9 and R10 residues and the phosphorus atoms on the surface, with internuclear distances of 4.62 +/- 0.29 A and 4.53 +/- 0.16 A, respectively. Conversely, results also indicate weak coupling between R13 and the surface, suggesting this residue is more removed from the surface than R9 and R10. Combining these results with previous data, a new model for the molecular recognition of HAP by statherin is constructed. |
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Authors:
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Moise Ndao; Jason T Ash; Patrick S Stayton; Gary P Drobny |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Surface science Volume: 604 ISSN: 0039-6028 ISO Abbreviation: - Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-8-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9876683 Medline TA: Surf Sci Country: - |
Other Details:
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Languages: ENG Pagination: L39-L42 Citation Subset: - |
Affiliation:
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Department of Chemistry, University of Washington, Seattle, Washington 98195. |
Export Citation:
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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R01 DE012554-06//NIDCR NIH HHS; R01 GM074511-03//NIGMS NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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