Document Detail


Role of angiopoietin-2 in corneal lymphangiogenesis.
MedLine Citation:
PMID:  24781940     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Lymphatic research has progressed rapidly in recent years. Lymphatic dysfunction has been found in myriad disorders from cancer metastasis to transplant rejection; however, effective treatment for lymphatic disorders is still limited. This study investigates the role of angiopoietin-2 (Ang-2) in corneal inflammatory lymphangiogenesis (LG) in vivo and in lymphatic endothelial cell (LEC) functions in vitro.
METHODS: Standard suture placement model was used to study Ang-2 expression in inflamed cornea, and corneal LG and hemangiogenesis (HG) responses in Ang-2 knockout mice. Moreover, human LEC culture system was used to examine the effect of Ang-2 gene knockdown on LEC functions using small interfering RNAs (siRNAs). The effect of siRNA treatment on corneal LG was also assessed in vivo.
RESULTS: Angiopoietin-2 was expressed on lymphatic vessels and macrophages in inflamed cornea. While corneal LG response was abolished in Ang-2 knockout mice, the HG response was also significantly suppressed with disorganized patterning. Moreover, anti-Ang-2 treatment inhibited LEC proliferation and capillary tube formation in vitro and corneal LG in vivo.
CONCLUSIONS: Angiopoietin-2 is critically involved in lymphatic processes in vivo and in vitro. Further investigation of the Ang-2 pathway may provide novel insights and therapeutic strategies for lymphatic-related disorders, which occur both inside and outside the eye.
Authors:
Don Yuen; Sammy Grimaldo; Roberto Sessa; Tatiana Ecoiffier; Tan Truong; Eric Huang; Michael Bernas; Sarah Daley; Marlys Witte; Lu Chen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2014-04-29
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  55     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-05-30     Completed Date:  2014-07-22     Revised Date:  2014-11-04    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3320-7     Citation Subset:  IM    
Copyright Information:
Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
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MeSH Terms
Descriptor/Qualifier:
Angiopoietin-2 / biosynthesis,  genetics*
Animals
Cornea / blood supply*,  metabolism,  pathology
Corneal Neovascularization / genetics*,  metabolism,  pathology
Disease Models, Animal
Endothelial Cells / metabolism,  pathology
Gene Expression Regulation*
Humans
Lymphangiogenesis / genetics*
Lymphatic Vessels / metabolism,  pathology*
Male
Mice
Mice, Inbred BALB C
Microscopy, Fluorescence
RNA / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
R01 EY017392/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Angiopoietin-2; 63231-63-0/RNA
Comments/Corrections

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