| Role of the ABC transporter Ste6 in cell fusion during yeast conjugation. | |
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MedLine Citation:
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PMID: 8909547 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Though early stages of yeast conjugation are well-mimicked by treatment with pheromones, the final degradation of the cell wall and membrane fusion of mating that leads to cytoplasmic mixing may require separate signals. Mutations that blocked cell fusion during mating in Saccharomyces cerevisiae were identified in a multipartite screen. The three tightest mutations proved to be partial-function alleles of the ABC-transporter gene STE6 required for transport of a-factor. The ste6(cefl-1) allele was recovered and sequenced. The ste6(cefl-1) allele contained a stop codon predicted to truncate Ste6 at amino acid residue 862 (of 1290). The ste6(cef) mutations reduced, but did not eliminate, expression of a-factor. Light and electron microscopy revealed that unlike ste6 null mutations which block mating before the formation of mating pairs, the ste6(cef) (cell fusion) alleles permitted early steps in mating to proceed normally but blocked at a late stage in conjugation where mating partners were encased by a single cell wall and separated by only a thin layer of cell wall material we term the fusion wall. Morphologically the prezygotes appeared symmetrical with successful cell wall fusion at the periphery of the region of cell contact. Responses to a-factor were efficiently induced in partner cells under mating conditions as expected given the symmetric appearance of the prezygotes. A strain expressing a ste6(K1093A) mutation that conferred export of a twofold to fourfold higher level of a-factor than ste6(cef) did not accumulate prezygotes during mating which could indicate a tight threshold of a-factor signaling required for mating. However, mating to an sst2 partner which has a greatly increased sensitivity to a-factor did not suppress the fusion defect of a ste6(cef) strain. Overexpression of the structural gene for a-factor also did not suppress the fusion defect. It is possible that a-factor or STE6 play more complex roles in cell fusion. |
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Authors:
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L Elia; L Marsh |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of cell biology Volume: 135 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1996-12-16 Completed Date: 1996-12-16 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 741-51 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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genetics,
physiology* Alleles Biological Transport Cell Wall / ultrastructure Chromosome Mapping Conjugation, Genetic / genetics* Fungal Proteins / genetics, metabolism, physiology* Glycoproteins* Lipoproteins / genetics, pharmacology, physiology Mutation Peptides / pharmacology Pheromones / pharmacology Reproduction Saccharomyces cerevisiae / chemistry, cytology*, drug effects, genetics, physiology Saccharomyces cerevisiae Proteins* Sequence Analysis, DNA |
| Grant Support | |
ID/Acronym/Agency:
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GM43365/GM/NIGMS NIH HHS; P30 CA 13330/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/ATP-Binding Cassette Transporters; 0/Fungal Proteins; 0/Glycoproteins; 0/Lipoproteins; 0/MFA2 protein, S cerevisiae; 0/Peptides; 0/Pheromones; 0/STE6 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 61194-02-3/mating factor |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 11 Year: 1996 Volume: 135 Issue: 3 First Page: 741 Last Page: 751 ID: 2121058 Publisher Id: 97066066 PubMed Id: 8909547 |
| Role of the ABC transporter Ste6 in cell fusion during yeast conjugation | |
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