Document Detail


Role of 20-HETE in mediating the effect of dietary K intake on the apical K channels in the mTAL.
MedLine Citation:
PMID:  11208597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have used the patch-clamp technique to study the effect of dietary K intake on the apical K channels in the medullary thick ascending limb (mTAL) of rat kidneys. The channel activity, defined by the number of channels in a patch and the open probability (NPo), of the 30- and 70-pS K channels, was 0.18 and 0.11, respectively, in the mTAL from rats on a K-deficient diet. In contrast, NPo of the 30- and 70-pS K channels increased to 0.60 and 0.80, respectively, in the tubules from animals on a high-K diet. The concentration of 20-hydroxyeicosatetraenoic acid (20-HETE) measured with gas chromatography-mass spectrometry was 0.8 pg/microg protein in the mTAL from rats on a high-K diet and increased significantly to 4.6 pg/microg protein in the tubules from rats on a K-deficient diet. Addition of N-methylsulfonyl-12,12-dibromododec-11-enamide (DDMS) or 17-octadecynoic acid (17-ODYA), agents that inhibit the formation of 20-HETE, had no significant effect on the activity of the 30-pS K channels. However, DDMS/17-ODYA significantly increased the activity of the apical 70-pS K channel from 0.11 to 0.91 in the mTAL from rats on a K-deficient diet. In contrast, inhibition of the cytochrome P-450 metabolism of arachidonic acid increased NPo from 0.64 to 0.81 in the tubules from animals on a high-K diet. Furthermore, the sensitivity of the 70-pS K channel to 20-HETE was the same between rats on a high-K diet and on a K-deficient diet. Finally, the pretreatment of the tubules with DDMS increased NPo of the 70-pS K channels in the mTAL from rats on a K-deficient diet to 0.76. We conclude that an increase in 20-HETE production is involved in reducing the activity of the apical 70-pS K channels in the mTAL from rats on a K-deficient diet.
Authors:
R Gu; Y Wei; H Jiang; M Balazy; W Wang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Renal physiology     Volume:  280     ISSN:  1931-857X     ISO Abbreviation:  Am. J. Physiol. Renal Physiol.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-03-06     Completed Date:  2001-03-29     Revised Date:  2011-04-28    
Medline Journal Info:
Nlm Unique ID:  100901990     Medline TA:  Am J Physiol Renal Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  F223-30     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, New York Medical College, Valhalla, New York 10595, USA.
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MeSH Terms
Descriptor/Qualifier:
Amides / pharmacology
Animals
Enzyme Inhibitors / pharmacology
Hydroxyeicosatetraenoic Acids / pharmacology*,  physiology
Kidney Tubules / drug effects*,  physiology
Potassium Channels / drug effects*,  physiology
Potassium, Dietary / pharmacology*
Rats
Rats, Sprague-Dawley
Sulfones / pharmacology
Grant Support
ID/Acronym/Agency:
DK-47402/DK/NIDDK NIH HHS; HL-34300/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Amides; 0/DDMS; 0/Enzyme Inhibitors; 0/Hydroxyeicosatetraenoic Acids; 0/Potassium Channels; 0/Potassium, Dietary; 0/Sulfones; 79551-86-3/20-hydroxy-5,8,11,14-eicosatetraenoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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