Document Detail

Rodent tracheal epithelial cells in vitro: a comparative study of normal cells, enhanced growth variants, SV-40 transformed cells and their interactions.
MedLine Citation:
PMID:  8920717     Owner:  NLM     Status:  MEDLINE    
Tracheal epithelial cells of the golden Syrian hamster can be successfully cultivated in vitro and applied as a model system of respiratory tract epithelium. By morphology and growth characteristics of tracheal epithelial cells, "normal cells", "enhanced growth variants" and "transformed cells" were distinguished in vitro. Hamster tracheal epithelial cells, transformed by Simian virus (SV)-40 expressed in cell nuclei the specific tumor (T-) antigen and showed an accumulation of tumor suppressor protein p53 by immunofluorescence. Cocultivation of "enhanced growth variants" and of "transformed cells" on a "feeder layer" of normal hamster tracheal epithelial cells revealed remarkable differences in loss of "contact inhibition of growth". A "cytokine" released by NIH-3T3 cells stimulated cell proliferation and seems to be important for cell growth of hamster tracheal epithelial cells. Preliminary characterization of the "cytokine" disclosed a molecular weight of more than 30 kDa, a relative thermostability and a loss of activity by treatment with mercaptoethanol indicating disulfide bridges in a molecule.
L Maciuleviciute; C Hornberg; N H Seemayer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Toxicology letters     Volume:  88     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1996-12-18     Completed Date:  1996-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  55-64     Citation Subset:  IM    
Medical Institute of Environmental Hygiene, Heinrich-Heine-University, Düsseldorf, Germany.
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MeSH Terms
3T3 Cells / physiology
Cell Division / drug effects
Cell Nucleus / chemistry
Cell Transformation, Viral*
Cells, Cultured / physiology
Coculture Techniques
Culture Media, Conditioned / pharmacology
Epithelial Cells
Epithelium / metabolism
Simian virus 40 / physiology
Trachea / cytology*,  metabolism
Tumor Suppressor Protein p53 / analysis
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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