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Rod photopigment kinetics after photo-disruption of the retinal pigment epithelium.
MedLine Citation:
PMID:  25316724     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Purpose: Advances in retinal imaging have led to the discovery of long-lasting retinal changes, including disruption of the retinal pigment epithelium (RPE) caused by light exposures below published safety limits. To investigate the functional consequences of RPE disruption, we combined adaptive optics ophthalmoscopy with retinal densitometry. Methods: A modified adaptive optics scanning light ophthalmoscope (AOSLO) measured the apparent density and regeneration rate of rhodopsin in two macaques before and after 4 different 568 nm retinal radiant exposures (RREs, 400 - 3200 J/cm2). OCT was used to measure the optical path length through the photoreceptor outer segments before and after RPE disruption. Results: All tested RREs caused visible RPE disruption. Apparent rhodopsin density was significantly reduced following 1600 J/cm2 (p = 0.01) and 3200 J/cm2 (p = 0.007) exposures. No significant change in apparent density was observed in response to 800 J/cm2. Surprisingly, exposure to 400 J/cm2 showed a significant increase in apparent density (p = 0.047). Rhodopsin recovery rate was not significantly affected by these RREs. OCT measurements showed a significant decrease in the optical path length through the photoreceptor outer segments for RREs above 800 J/cm2 (p < 0.001). Conclusions: At higher RREs, optical path length through the outer segments was reduced. However, the rate of photopigment regeneration was unchanged. While some ambiguity remains as to the correlation between measured reflectivity and absolute rhodopsin density; at the lowest RREs, RPE disruption appears not to be accompanied by a loss of apparent rhodopsin density, which would have been indicative of functional loss.
Authors:
Benjamin D Masella; Jennifer J Hunter; David R Williams
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-10-14
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  -     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-15     Completed Date:  -     Revised Date:  2014-10-16    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 by Association for Research in Vision and Ophthalmology.
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