| Rod, M-cone and M/S-cone inputs to hyperpolarizing bipolar cells in the mouse retina. | |
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MedLine Citation:
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PMID: 22219344 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bipolar cells are the central neurons of the retina that convey visual signals from rod and cone photoreceptors in the outer retina to higher-order neurons in the inner retina and the brain. Early anatomical studies have suggested that there are four types of cone hyperpolarizing (OFF) bipolar cells (HBCs) in the mouse retina, but no light responses have been systematically examined. By analysing light-evoked cation and chloride currents (I(C) and I(Cl)) from over 50 morphologically identified HBCs in the dark-adapted wildtype and connexin36 knockout (Cx36(-/-)) mouse retinas, we identified three types of HBCs, each with distinct light responses and morphological characteristics. The HBC(R/MC)s with axon terminals ramifying between 0% and 30% of the inner plexiform layer (IPL) receive mixed inputs from rods and M-cones, the HBC(MC)s with axon terminals ramifying between 10% and 50% of the IPL receive inputs primarily from M-cones, and the HBC(M/SC)s with axon terminals ramifying between 25% and 50% of IPL receive inputs primarily from cones with mixed M- and S-cone pigments. Moreover, we found that HBC(R/MC)s in the Cx36(-/-) mice exhibit light responses very similar to the wildtype HBC(R/MC)s, suggesting that the mixed rod-cone inputs are not mediated by connexin36-dependent rod-cone coupling, but rather by direct synaptic contacts from rods and M-cones. This study constitutes the first systematic investigation that correlates light response characteristics and axonal morphology of HBCs in dark-adapted mouse retina, and contributes to recently emerging evidence that revises the traditional view that mammalian HBCs only contact cone photoreceptors. |
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Authors:
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Ji-Jie Pang; Fan Gao; David L Paul; Samuel M Wu |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-01-04 |
Journal Detail:
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Title: The Journal of physiology Volume: 590 ISSN: 1469-7793 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-02-20 Completed Date: 2012-06-26 Revised Date: 2013-04-12 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 845-54 Citation Subset: IM |
Affiliation:
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Cullen Eye Institute, Baylor College of Medicine, One Baylor Plaza, NC-205, Houston, TX 77030, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Connexins / deficiency, genetics Light Mice Mice, Inbred C57BL Mice, Knockout Retinal Cone Photoreceptor Cells / physiology* Retinal Rod Photoreceptor Cells / physiology* Synapses / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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EY004446/EY/NEI NIH HHS; EY014127/EY/NEI NIH HHS; EY019908/EY/NEI NIH HHS; EY02520/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Connexins; 0/connexin 36 |
| Comments/Corrections | |
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