| Robust utilization of phospholipase-generated metabolites, glycerophosphodiesters, by Candida albicans: Role of the CaGit1 permease. | |
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MedLine Citation:
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PMID: 21984707 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Glycerophosphodiesters are the products of phospholipase-mediated deacylation of phospholipids. In Saccharomyces cerevisiae, a single gene, GIT1, encodes a permease responsible for importing glycerophosphodiesters, such as glycerophosphoinositol and glycerophosphocholine into the cell. In contrast, the Candida albicans genome contains four ORFs with a high degree of similarity to ScGit1. Here, we report that C. albicans utilizes glycerophosphoinositol and glycerophosphocholine as sources of phosphate at both mildly acidic and physiological pH. Insertional mutagenesis of CaGIT1 (orf19.34), the ORF most similar to ScGit1, abolished the ability of cells to use GroPIns as a phosphate source at acidic pH and to transport [(3)H]GroPIns at acidic and physiological pH, while reintegration of a GIT1 allele into the genome restored those functions. Several lines of evidence, including the detection of internal [(3)H]GroPIns, indicated that GroPIns is transported intact through CaGit1. GroPIns transport was shown to conform to Michaelis-Menton kinetics, with an apparent K(m) = 28 ± 6 μM. Notably, uptake of label from [(3)H]GroPCho was found to be roughly 50-fold greater than uptake of label from [(3)H]GroPIns, and roughly 500-fold greater than the equivalent activity in S. cerevisiae. Insertional mutagenesis of CaGIT1 had no effect on the utilization of GroPCho as a phosphate source or the uptake of label from [(3)H]GroPCho. Growth under low phosphate conditions was shown to increase label uptake from both [(3)H]GroPIns and [(3)H]-GroPCho. Screening of a transcription factor deletion set identified CaPHO4 as required for the utilization of GroPIns, but not GroPCho, as a phosphate source. |
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Authors:
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Andrew C Bishop; Tao Sun; Mitchell E Johnson; Vincent M Bruno; Jana Patton-Vogt |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-7 |
Journal Detail:
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Title: Eukaryotic cell Volume: - ISSN: 1535-9786 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101130731 Medline TA: Eukaryot Cell Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15283. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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