Document Detail


Robust nanobioconjugates of Candida antarctica lipase B - Multiwalled carbon nanotubes: Characterization and application for multiple usages in non-aqueous biocatalysis.
MedLine Citation:
PMID:  23685646     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Present work elucidates two approaches for covalent attachment of the enzyme Candida antarctica lipase B (CALB) on multiwalled carbon nanotubes (MWCNTs). One method of enzyme immobilization involved carbodiimide chemistry while in the other approach, the cross linker (3-Aminopropyl) triethoxysilane (APTES) followed by succinic acid anhydride (SAA) were employed prior to carbodiimide activation. Modified MWCNTs were characterized by transmission electron microscopy (TEM), Fourier transformation infrared spectroscopic (FTIR), Raman spectroscopy and thermal gravitometric analysis (TGA). The lipase-MWCNTs conjugates were applied for synthesis of the flavor ester 'pentyl valerate' in cyclohexane and effects of solvent, temperature and agitation on ester synthesis were studied. Upon subject to reusability studies for 50 cycles, the bionanoconjugates were found to be highly sturdy and exhibited ∼79% activity (immobilization using carbodiimide) whereas the nanoconjugate prepared using APTES and SAA retained only up to ∼30% activity.
Authors:
Tripti Raghavendra; Arpana Basak; Lalit M Manocha; Amita R Shah; Datta Madamwar
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-4-28
Journal Detail:
Title:  Bioresource technology     Volume:  140C     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-5-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  103-110     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Elsevier Ltd. All rights reserved.
Affiliation:
BRD School of Biosciences, Satellite Campus, Vadtal Road, P.O. Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India. Electronic address: raghavendra.tripti@gmail.com.
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