| Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase. | |
| | |
MedLine Citation:
|
PMID: 20828122 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Firefly luciferase utilizes the chemical energy of ATP and oxygen to convert its substrate, D-luciferin, into an excited-state oxyluciferin molecule. Relaxation of this molecule to the ground state is responsible for the yellow-green light emission. Synthetic cyclic alkylaminoluciferins that allow robust red-shifted light emission with the modified luciferase Ultra-Glo are described. Overall light emission is higher than that of acyclic alkylaminoluciferins, aminoluciferin, and the native substrate D-luciferin. |
| | |
Authors:
|
Gadarla Randheer Reddy; Walter C Thompson; Stephen C Miller |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Journal of the American Chemical Society Volume: 132 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-09-30 Completed Date: 2011-01-20 Revised Date: 2012-01-19 |
Medline Journal Info:
|
Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
|
Languages: eng Pagination: 13586-7 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
chemistry Animals Benzothiazoles / chemistry Fireflies / enzymology* Firefly Luciferin / chemical synthesis, chemistry* Light* Luciferases, Firefly / chemistry, metabolism* Luminescent Agents / chemical synthesis, chemistry* Molecular Structure Oxygen / chemistry Stereoisomerism Substrate Specificity |
| Grant Support | |
ID/Acronym/Agency:
|
R21 CA127196-02/CA/NCI NIH HHS; R21CA127196/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Benzothiazoles; 0/D-luciferin; 0/Luminescent Agents; 2591-17-5/Firefly Luciferin; 56-65-5/Adenosine Triphosphate; 7782-44-7/Oxygen; EC 1.13.12.7/Luciferases, Firefly |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Electrochemical quartz crystal microbalance (EQCM) studies of ions and solvents insertion into highl...
Next Document: Mixed low-dimensional nanomaterial: 2D ultranarrow MoS2 inorganic nanoribbons encapsulated in quasi-...