Document Detail

Risk reduction of ethyl acetate fraction of Empetrum nigrum var. japonicum via antioxidant properties against hydrogen peroxide-induced cell damage.
MedLine Citation:
PMID:  20077224     Owner:  NLM     Status:  MEDLINE    
Reactive oxygen species (ROS) produce damage to all major cellular constituents. The antioxidant properties of the ethyl acetate fraction of Empetrum nigrum was assessed against hydrogen peroxide (H(2)O(2))-induced cell damage. Empetrum extract was found to scavenge (1) intracellular ROS in cell system, (2) hydroxyl radicals generated by the Fenton reaction (FeSO(4) + H(2)O(2)), and (3) superoxide radicals generated by xanthine/xanthine oxidase in a cell-free system as detected by electron spin resonance (ESR) spectrometry. Cell damage was produced by H(2)O(2) treatment as evidenced by DNA damage, lipid peroxidation, and increased protein carbonyl formation; however, Empetrum extract prevented H(2)O(2)-induced damage to these parameters. Empetrum extract increased viability of Chinese hamster lung fibroblast (V79-4) cells exposed to H(2)O(2), as evidenced by decreased apoptotic nuclear fragmentation, and lower sub G(1) cell population. Further, Empetrum extract restored the cellular antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and heme oxygenase-1 (HO-1), which were reduced by H(2)O(2) treatment. In conclusion, Empetrum extract protected cells against H(2)O(2)-induced cell damage via antioxidant properties by scavenging ROS and enhancing antioxidant enzyme activities.
Ki Cheon Kim; Kyoung Ah Kang; Rui Zhang; Mei Jing Piao; Young Jun Heo; Sungwook Chae; Gi Young Kim; Ji Young Moon; Byoung Sam Yoo; Jin Won Hyun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of toxicology and environmental health. Part A     Volume:  72     ISSN:  1528-7394     ISO Abbreviation:  J. Toxicol. Environ. Health Part A     Publication Date:  2009  
Date Detail:
Created Date:  2010-01-15     Completed Date:  2010-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100960995     Medline TA:  J Toxicol Environ Health A     Country:  England    
Other Details:
Languages:  eng     Pagination:  1499-508     Citation Subset:  IM    
School of Applied Marine Science, Jeju National University, Jeju-si, Korea.
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MeSH Terms
Acetates / chemistry*
Cell Line
Cytoprotection / drug effects
Ericaceae / chemistry*
Fibroblasts / drug effects*
Hydrogen Peroxide / toxicity*
Oxidative Stress / drug effects
Plant Extracts / chemistry,  pharmacology*
Reg. No./Substance:
0/Acetates; 0/Plant Extracts; 141-78-6/ethyl acetate; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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