Document Detail


Right ventricular systolic pressure measurements in combination with harvest of lung and immune tissue samples in mice.
MedLine Citation:
PMID:  23354416     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The function of the right heart is to pump blood through the lungs, thus linking right heart physiology and pulmonary vascular physiology. Inflammation is a common modifier of heart and lung function, by elaborating cellular infiltration, production of cytokines and growth factors, and by initiating remodeling processes. Compared to the left ventricle, the right ventricle is a low-pressure pump that operates in a relatively narrow zone of pressure changes. Increased pulmonary artery pressures are associated with increased pressure in the lung vascular bed and pulmonary hypertension. Pulmonary hypertension is often associated with inflammatory lung diseases, for example chronic obstructive pulmonary disease, or autoimmune diseases. Because pulmonary hypertension confers a bad prognosis for quality of life and life expectancy, much research is directed towards understanding the mechanisms that might be targets for pharmaceutical intervention. The main challenge for the development of effective management tools for pulmonary hypertension remains the complexity of the simultaneous understanding of molecular and cellular changes in the right heart, the lungs and the immune system. Here, we present a procedural workflow for the rapid and precise measurement of pressure changes in the right heart of mice and the simultaneous harvest of samples from heart, lungs and immune tissues. The method is based on the direct catheterization of the right ventricle via the jugular vein in close-chested mice, first developed in the late 1990s as surrogate measure of pressures in the pulmonary artery. The organized team-approach facilitates a very rapid right heart catheterization technique. This makes it possible to perform the measurements in mice that spontaneously breathe room air. The organization of the work-flow in distinct work-areas reduces time delay and opens the possibility to simultaneously perform physiology experiments and harvest immune, heart and lung tissues. The procedural workflow outlined here can be adapted for a wide variety of laboratory settings and study designs, from small, targeted experiments, to large drug screening assays. The simultaneous acquisition of cardiac physiology data that can be expanded to include echocardiography and harvest of heart, lung and immune tissues reduces the number of animals needed to obtain data that move the scientific knowledge basis forward. The procedural workflow presented here also provides an ideal basis for gaining knowledge of the networks that link immune, lung and heart function. The same principles outlined here can be adapted to study other or additional organs as needed.
Authors:
Wen-Chi Chen; Sung-Hyun Park; Carol Hoffman; Cecil Philip; Linda Robinson; James West; Gabriele Grunig
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media     Date:  2013-01-16
Journal Detail:
Title:  Journal of visualized experiments : JoVE     Volume:  -     ISSN:  1940-087X     ISO Abbreviation:  J Vis Exp     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-28     Completed Date:  2013-03-17     Revised Date:  2014-01-23    
Medline Journal Info:
Nlm Unique ID:  101313252     Medline TA:  J Vis Exp     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e50023     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cardiac Surgical Procedures / methods*
Female
Heart / physiology*
Lung / immunology,  physiology*,  surgery*
Lymph Nodes / immunology,  surgery*
Male
Mice
Spleen / immunology,  surgery*
Systole / physiology
Ventricular Function, Right / physiology
Grant Support
ID/Acronym/Agency:
1R01 HL095764-01/HL/NHLBI NIH HHS; 1R21HL092370-01/HL/NHLBI NIH HHS; R01 HL095764/HL/NHLBI NIH HHS; R01HL082694/HL/NHLBI NIH HHS; R21 HL092370/HL/NHLBI NIH HHS
Comments/Corrections

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