Document Detail


Right ventricular outflow tract repair with a cardiac biologic scaffold.
MedLine Citation:
PMID:  22025093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Surgical reconstruction of congenital heart defects is often limited by the nonresorbable material used to approximate normal anatomy. In contrast, biologic scaffold materials composed of resorbable non-cross-linked extracellular matrix (ECM) have been used for tissue reconstruction of multiple organs and are replaced by host tissue. Preparation of whole organ ECM by decellularization through vascular perfusion can maintain much of the native three-dimensional (3D) structure, strength, and tissue-specific composition. A 3D cardiac ECM (C-ECM) biologic scaffold material would logically have structural and functional advantages over materials such as Dacron™ for myocardial repair, but the in vivo remodeling characteristics of C-ECM have not been investigated to date.
METHODS AND RESULTS: A porcine C-ECM patch or Dacron patch was used to reconstruct a full-thickness right ventricular outflow tract (RVOT) defect in a rat model with end points of structural remodeling function at 16 weeks. The Dacron patch was encapsulated by dense fibrous tissue and showed little cellular infiltration. Echocardiographic analysis showed that the right ventricle of the hearts patched with Dacron were dilated at 16 weeks compared to presurgery baseline values. The C-ECM patch remodeled into dense, cellular connective tissue with scattered small islands of cardiomyocytes. The hearts patched with C-ECM showed no difference in the size or function of the ventricles as compared to baseline values at both 4 and 16 weeks.
CONCLUSIONS: The C-ECM patch was associated with better functional and histomorphological outcomes compared to the Dacron patch in this rat model of RVOT reconstruction.
Authors:
John M Wainwright; Ryotaro Hashizume; Kazuro L Fujimoto; Nathaniel T Remlinger; Colin Pesyna; William R Wagner; Kimimasa Tobita; Thomas W Gilbert; Stephen F Badylak
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-10-24
Journal Detail:
Title:  Cells, tissues, organs     Volume:  195     ISSN:  1422-6421     ISO Abbreviation:  Cells Tissues Organs (Print)     Publication Date:  2012  
Date Detail:
Created Date:  2011-12-19     Completed Date:  2012-04-09     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  100883360     Medline TA:  Cells Tissues Organs     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  159-70     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 S. Karger AG, Basel.
Affiliation:
Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pa., USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Echocardiography
Extracellular Matrix / chemistry*
Female
Heart Defects, Congenital / pathology,  surgery
Heart Ventricles / cytology*,  pathology,  surgery*
Polyethylene Terephthalates / chemistry
Rats
Rats, Inbred Lew
Swine
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
Grant Support
ID/Acronym/Agency:
1R03EB009237-01A1/EB/NIBIB NIH HHS; R03 EB009237-01/EB/NIBIB NIH HHS; T32 HL76124/HL/NHLBI NIH HHS; T32-HL76124/HL/NHLBI NIH HHS; UL1 RR024153/RR/NCRR NIH HHS; UL1 TR000005/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Polyethylene Terephthalates
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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