| Rickettsia prowazekii requires host cell serine and glycine for growth. | |
| | |
MedLine Citation:
|
PMID: 3098686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The growth requirement of Rickettsia prowazekii for the amino acids serine and glycine was assessed in both wild-type cell lines and a mutant cell line. X-irradiated L929 cells supported the growth of R. prowazekii when the cells were incubated in Eagle minimal essential medium supplemented with serum. In contrast, in this medium, X-irradiated Vero cells did not support the growth of rickettsiae unless cycloheximide, serine, or glycine was added. Other nonessential amino acids, additional glucose, and potential products of host cell metabolism of serine and glycine were nonstimulatory. The concentration of serine or glycine required to support rickettsial growth had no effect on the doubling time of uninfected, unirradiated Vero cells. A comparison of intracellular amino acid pools indicated that the serine and glycine concentrations in mock-infected Vero cells were approximately 31 and 14% of the respective concentrations in mock-infected L929 cells. The pools of both amino acids in Vero cells increased markedly upon treatment of the cells with cycloheximide. Interconversion of serine and glycine catalyzed by serine hydroxymethyltransferase was detected in cell-free extracts of purified rickettsiae. However, this enzymatic activity did not permit rickettsial growth in a glycine-requiring clone (772-56d) of the Chinese hamster ovary cell CHO-K1 in the absence of glycine supplementation. These data indicate that R. prowazekii depends on the host cell for serine or glycine. |
| | |
Authors:
|
F E Austin; J Turco; H H Winkler |
Related Documents
:
|
190156 - Some biological properties of herpesvirus saimiri from chronically infected monolayer a... 1391686 - Changes in membrane permeability during semliki forest virus induced cell fusion. 6329966 - Isolation of htlv derived from japanese adult t-cell leukemia patients in human diploid... 15196816 - The low molecular weight heparan sulfate-mimetic, pi-88, inhibits cell-to-cell spread o... 11295536 - Monochloramine inhibits etoposide-induced apoptosis with an increase in dna aberration. 21403466 - P27(kip1) is required to maintain proliferative quiescence in the adult cochlea and pit... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Infection and immunity Volume: 55 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1987 Jan |
Date Detail:
|
Created Date: 1987-02-05 Completed Date: 1987-02-05 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 240-4 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
metabolism Animals Glycine / metabolism* L Cells (Cell Line) Rickettsia prowazekii / growth & development, metabolism* Serine / metabolism* Vero Cells |
| Grant Support | |
ID/Acronym/Agency:
|
AI07031/AI/NIAID NIH HHS; AI15035/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 56-40-6/Glycine; 56-45-1/Serine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation of Coccidioides immitis F antigen by immunoaffinity chromatography with monospecific antis...
Next Document: Characterization of protein I from serum-sensitive and serum-resistant transformants of Neisseria go...