Document Detail

Rickettsia prowazekii requires host cell serine and glycine for growth.
MedLine Citation:
PMID:  3098686     Owner:  NLM     Status:  MEDLINE    
The growth requirement of Rickettsia prowazekii for the amino acids serine and glycine was assessed in both wild-type cell lines and a mutant cell line. X-irradiated L929 cells supported the growth of R. prowazekii when the cells were incubated in Eagle minimal essential medium supplemented with serum. In contrast, in this medium, X-irradiated Vero cells did not support the growth of rickettsiae unless cycloheximide, serine, or glycine was added. Other nonessential amino acids, additional glucose, and potential products of host cell metabolism of serine and glycine were nonstimulatory. The concentration of serine or glycine required to support rickettsial growth had no effect on the doubling time of uninfected, unirradiated Vero cells. A comparison of intracellular amino acid pools indicated that the serine and glycine concentrations in mock-infected Vero cells were approximately 31 and 14% of the respective concentrations in mock-infected L929 cells. The pools of both amino acids in Vero cells increased markedly upon treatment of the cells with cycloheximide. Interconversion of serine and glycine catalyzed by serine hydroxymethyltransferase was detected in cell-free extracts of purified rickettsiae. However, this enzymatic activity did not permit rickettsial growth in a glycine-requiring clone (772-56d) of the Chinese hamster ovary cell CHO-K1 in the absence of glycine supplementation. These data indicate that R. prowazekii depends on the host cell for serine or glycine.
F E Austin; J Turco; H H Winkler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  55     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-02-05     Completed Date:  1987-02-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  240-4     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism
Glycine / metabolism*
L Cells (Cell Line)
Rickettsia prowazekii / growth & development,  metabolism*
Serine / metabolism*
Vero Cells
Grant Support
Reg. No./Substance:
0/Amino Acids; 56-40-6/Glycine; 56-45-1/Serine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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