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Rickettsia parkeri and Candidatus Rickettsia andeanae in Gulf Coast ticks, Mississippi, USA.
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MedLine Citation:
PMID:  23018026     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Authors:
Flavia A G Ferrari; Jerome Goddard; Christopher D Paddock; Andrea S Varela-Stokes
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Publication Detail:
Type:  Letter; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Emerging infectious diseases     Volume:  18     ISSN:  1080-6059     ISO Abbreviation:  Emerging Infect. Dis.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-02-19     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  9508155     Medline TA:  Emerg Infect Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1705-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA, Mitochondrial / genetics
Ixodidae / classification,  genetics,  microbiology*
Mississippi
Prevalence
RNA, Ribosomal, 16S / genetics
Rickettsia / classification,  genetics*,  isolation & purification*
Rickettsia Infections / transmission
Chemical
Reg. No./Substance:
0/DNA, Mitochondrial; 0/RNA, Ribosomal, 16S
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Emerg Infect Dis
Journal ID (iso-abbrev): Emerging Infect. Dis
Journal ID (publisher-id): EID
ISSN: 1080-6040
ISSN: 1080-6059
Publisher: Centers for Disease Control and Prevention
Article Information
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Print publication date: Month: 10 Year: 2012
Volume: 18 Issue: 10
First Page: 1705 Last Page: 1707
PubMed Id: 23018026
ID: 3471625
Publisher Id: 12-0250
DOI: 10.3201/eid1810.120250

Rickettsia parkeri and Candidatus Rickettsia andeanae in Gulf Coast Ticks, Mississippi, USA Alternate Title:R. parkeri and Candidatus R. andeanae in Ticks
Flavia A.G. Ferrari
Jerome Goddard
Christopher D. Paddock
Andrea S. Varela-Stokes
Mississippi State University, Mississippi State, Mississippi, USA (F.A.G. Ferrari, J. Goddard, A.S. Varela-Stokes);
and Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C.D. Paddock)
Correspondence: Address for correspondence: Andrea S. Varela-Stokes, Mississippi State University, Wise Center, 240 Wise Center Dr, Mississippi State, MS 39762, USA; email: stokes@cvm.msstate.edu

To the Editor:Rickettsia parkeri, a spotted fever group Rickettsia (SFGR) bacterium, is transmitted by Amblyomma maculatum, the Gulf Coast tick (1). The prevalence of R. parkeri in Gulf Coast ticks has been reported as <42% in the United States, which is higher than reported rates of R. rickettsii (the cause of Rocky Mountain spotted fever) in Dermacentor species ticks. Misdiagnosis among SFGR infections is not uncommon, and R. parkeri rickettsiosis can cause symptoms similar to those for mild Rocky Mountain spotted fever (1). We evaluated infection rates of R. parkeri and Candidatus Rickettsia andeanae, a recently identified but incompletely characterized SFGR, in Gulf Coast ticks in Mississippi, USA.

During May–September 2008–2010, we collected adult Gulf Coast ticks from vegetation at 10 sites in Mississippi. We extracted genomic DNA from the ticks using the illustra tissue and cells genomicPrep Mini Spin Kit (GE Healthcare Life Sciences, Piscataway, NJ, USA). We tested amplifiable tick DNA by PCR of the tick mitochondrial 16S rRNA gene (2). We tested for molecular evidence of any SFGR species by nested PCR of rompA (rickettsial outer membrane protein A gene) (1). Samples positive for SFGR were subsequently tested by using species-specific rompA PCR for R. parkeri (3) and Candidatus R. andeanae (4). All PCRs included 1) a positive control of DNA from cultured R. parkeri– (Tate’s Hell strain) or Candidatus R. andeanae–infected Gulf Coast ticks and 2) a negative control of water (nontemplate). PCR products were purified by using Montage PCR Centrifugal Filter Devices (Millipore, Bedford, MA, USA) and sequenced by using Eurofins MWG Operon (Huntsville, AL, USA). We generated consensus sequences using ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/) alignment and identified the sequences using GenBank BLAST searches (www.ebi.ac.uk/Tools/clustalw2/).

Proportions of ticks infected with SFGR, by region and year, were compared separately by using Fisher exact test followed by pairwise comparisons with a Bonferroni adjustment (PROC FREQ, SAS for Windows, V9.2; SAS Institute, Cary, NC, USA). For all analyses, p<0.05 was considered significant. An index of co-infection was calculated by using the formula IC = ([O – E]/N) × 100, in which IC is index of co-infection, O is number of co-infections, E is expected occurrence of co-infection caused by chance alone, and N is total number of ticks infected by either or both Rickettsia species. A χ2 test was used to determine statistical significance (5).

A total of 707 adult Gulf Coast ticks were collected during the 3 years (350 in 2008, 194 in 2009, and 163 in 2010). Tick mitochondrial 16S rRNA gene was detected in 698 (98.7%), of which 128 (18.3%) were positive for SFGR DNA, comprising 106 (15.2%) positive only for R. parkeri, 10 (1.4%) positive only for Candidatus R. andeanae, and 12 (1.7%) co-infected with R. parkeri and Candidatus R. andeanae (Table). Positive test results from 22 ticks singly or co-infected with Candidatus R. andeanae were confirmed by sequencing.

Most (94.6%) ticks were from northern (n = 260) and southern (n = 409) Mississippi (Technical Appendix Figure). No significant difference in the number of R. parkeri–infected ticks between northern and southern Mississippi was observed (p = 0.13) (Table). However, significantly more ticks were singly infected with Candidatus R. andeanae in southern sites than in northern sites (p = 0.03). The infection rate for co-infected ticks in southern sites was higher than that in northern sites (p = 0.06). Among the 3 collection years for northern and southern sites, only the prevalence of R. parkeri in singly infected ticks differed significantly (p = 0.01) (data not shown); the infection rate was significantly greater during 2010 than during 2009 (p = 0.003, α/3 = 0.02). The overall index of co-infection with R. parkeri and Candidatus R. andeanae was 6.5, statistically higher than expected by chance alone (Table) (p<0.0001).

The overall prevalence of infection with SFGR species in Gulf Coast ticks sampled was 18.3%; 15.2% of ticks were singly infected with R. parkeri, and 1.7% were infected with R. parkeri and Candidatus R. andeanae. As reported, the frequency of R. parkeri in Gulf Coast ticks is generally high, ranging from ≈10% to 40% (3,4,68). We found approximately 1 R. parkeri-infected Gulf Coast tick for every 6 ticks tested, suggesting that infected Gulf Coast ticks are commonly encountered in Mississippi. Because Gulf Coast ticks are among the most common human-biting ticks in Mississippi (9), awareness of R. parkeri rickettsiosis should be increased in this state. We identified Candidatus R. andeanae in ≈3% of Gulf Coast ticks in Mississippi; this frequency is similar to those reported in other studies of Gulf Coast ticks in the southern United States (4,6). Our finding of co-infected Gulf Coast ticks is at a frequency significantly higher than expected from chance alone. The biologic role of co-infections of Gulf Coast ticks with R. parkeri and Candidatus R. andeanae remains to be determined.

Technical Appendix

Distribution of Gulf Coast ticks and cases of rickettsiosis.



Notes

Suggested citation for this article: Ferrari FAG, Goddard J, Paddock CD, Varela-Stokes AS. Rickettsia parkeri and CandidatusRickettsia andeanae in Gulf Coast ticks, Mississippi, USA [letter]. Emerg Infect Dis [Internet]. 2012 Oct [date cited]. http://dx.doi.org/10.3201/eid1810.120250

Acknowledgments

We acknowledge Gail Moraru, Diana Link, Claudenir Ferrari, Marcia Carvalho, and Ryan Lawrence for assisting with tick collection; Robert Wills for assisting with statistical analyses; and Erle Chenney and Whitney Smith for contributing to the molecular analysis of ticks.

This work was supported by a Southeastern Center for Emerging Biologic Threats grant awarded to A.S.V.-S. in 2009 and funding from the College of Veterinary Medicine at Mississippi State University.


References
1. . PaddockCD, SumnerJW, ComerJA, ZakiSR, GoldsmithCS, GoddardJ, et al. Rickettsia parkeri: a newly recognized cause of spotted fever rickettsiosis in the United States.Clin Infect Dis. Year: 2004;38:805–1110.1086/38189414999622
2. . BlackWC, KlompenJS, KeiransJEPhylogenetic relationships among tick subfamilies (Ixodida: Ixodidae: Argasidae) based on the 18S nuclear rDNA gene.Mol Phylogenet Evol. Year: 1997;7:129–4410.1006/mpev.1996.03829007027
3. . Varela-StokesAS, PaddockCD, EngberBMTRickettsia parkeri in Amblyomma maculatum ticks, North Carolina, USA, 2009–2010.Emerg Infect Dis. Year: 2011;17:2350–310.3201/eid1712.11078922172164
4. . PaddockCD, FournierPE, SumnerJW, GoddardJ, ElshenawyY, MetcalfeMG, et al. Isolation of Rickettsia parkeri and identification of a novel spotted fever group Rickettsia sp. from Gulf Coast ticks (Amblyomma maculatum) in the United States.Appl Environ Microbiol. Year: 2010;76:2689–9610.1128/AEM.02737-0920208020
5. . GinsbergHSPotential effects of mixed infections in ticks on transmission dynamics of pathogens: comparative analysis of published records.Exp Appl Acarol. Year: 2008;46:29–4110.1007/s10493-008-9175-518648996
6. . SumnerJW, DurdenLA, GoddardJ, StromdahlEY, ClarkKL, ReevesWK, et al. Gulf Coast ticks (Amblyomma maculatum) and Rickettsia parkeri, United States.Emerg Infect Dis. Year: 2007;13:751–310.3201/eid1305.06146817553257
7. . FornadelCM, ZhangX, SmithJD, PaddockCD, AriasJR, NorrisDEHigh rates of Rickettsia parkeri infection in Gulf Coast ticks (Amblyomma maculatum) and identification of "Candidatus Rickettsia andeanae" from Fairfax County, Virginia.Vector Borne Zoonotic Dis. Year: 2011;11:1535–910.1089/vbz.2011.065421867421
8. . WrightCL, NadolnyRM, JiangJ, RichardsAL, SonenshineDE, GaffHD, et al. Rickettsia parkeri in Gulf Coast ticks, Southeastern Virginia, USA.Emerg Infect Dis. Year: 2011;17:896–810.3201/eid1705.10183621529406
9. . GoddardJA ten-year study of tick biting in Mississippi: implications for human disease transmission.J Agromed. Year: 2002;8:25–3210.1300/J096v08n02_0612853269

Tables
[TableWrap ID: T1] Table  PCR results for adult Rickettsia parkeri– and Candidatus Rickettsia andeanae–infected Gulf Coast ticks (Amblyomma maculatum) collected from 10 sites in Mississippi, USA, 2008–2010*
Location (no. collection sites) No. ticks No. (%; 95% CI) SFG rompA No. (%; 95% CI) R. parkeri only No. (%; 95% CI) Candidatus R. andeanae only Expected no. (%) co-infected ticks No. (%; 95% CI) co-infected ticks
North (4) 257 49 (19.1; 14.5–24.4)† 48 (18.7; 14.1–24)‡ 0.19 (0.07) 1 (0.4; 0–2.1)¶
Central (1) 38 4 (10.5; NA) 1 (2.6; NA) 2 (5.3; NA) 0.16 (0.42) 1 (2.6; NA)
South (5) 403 75 (18.6; 14.9–22.8)† 57 (14.1; 10.9–17.9)‡ 8 (2.0; 0.9–3.9)§ 2.99 (0.74) 10 (2.5; 1.2–4.5)¶
Total (10) 698 128 (18.3; NA) 106 (15.2; NA) 10 (1.4; NA) 3.65 (0.52) 12 (1.7; NA)

*The estimated value of co-infection caused by chance alone (E) was calculated by using the formula E = (a + b)(a + c) / (a + b + c + d) (5), where a = no. ticks infected with both Rickettsia species, b = no. ticks infected only with R. parkeri, c = no. ticks infected only with Candidatus R. andeanae, and d = no. ticks not infected with either Rickettsia species. SFG rompA, spotted fever group rickettsial outer membrane protein A gene. NA, not applicable.
†p = 0.9187.
‡p = 0.1275.
§p = 0.0257.
¶p = 0.0578 (comparison of prevalence from northern and southern sites only).



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Keywords: Keywords: Amblyomma maculatum, co-infection, Mississippi, spotted fever group rickettsiae, Rickettsia parkeri, Candidatus Rickettsia andeanae, bacteria, Gulf Coast ticks, Rickettsia, United States, ticks, vector-borne infections.

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