Document Detail

Rice tungro bacilliform virus: transcription and translation in protoplasts.
MedLine Citation:
PMID:  8091688     Owner:  NLM     Status:  MEDLINE    
Protoplasts from cell suspension cultures of Oryza sativa (monocot) and Orychophragmus violaceus (dicot) support transcription from the rice tungro bacilliform virus (RTBV) promoter and translation of the resulting mRNA despite the presence of a long leader sequence with strong secondary structure and 12 short open reading frames. Transcriptional elements located both upstream and downstream of the transcription initiation site are defined by deletion analysis and the functional TATA motif is determined. Expression of an open reading frame downstream of the entire leader is more efficient than that downstream of truncated derivatives. For optimal expression sequences in the 5' and 3' parts of the leader are required.
G Chen; M Müller; I Potrykus; T Hohn; J Fütterer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virology     Volume:  204     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-10-20     Completed Date:  1994-10-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  91-100     Citation Subset:  IM    
Friedrich Miescher Institut, Basel, Switzerland.
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MeSH Terms
Base Sequence
Gene Expression Regulation, Viral / genetics*
Genome, Viral*
Molecular Sequence Data
Nucleic Acid Conformation
Open Reading Frames
Plant Viruses / genetics*
Plants / microbiology
Promoter Regions, Genetic / genetics
Protein Biosynthesis
Protoplasts / microbiology*
RNA, Messenger / genetics*
RNA, Viral / genetics
Recombinant Fusion Proteins / biosynthesis
Sequence Deletion / physiology
Transcription, Genetic
Reg. No./Substance:
0/RNA, Messenger; 0/RNA, Viral; 0/Recombinant Fusion Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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