Document Detail


Rice shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells.
MedLine Citation:
PMID:  17586689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We screened a rice (Oryza sativa L. 'Nipponbare') full-length cDNA expression library through functional complementation in yeast (Saccharomyces cerevisiae) to find novel cation transporters involved in salt tolerance. We found that expression of a cDNA clone, encoding the rice homolog of Shaker family K(+) channel KAT1 (OsKAT1), suppressed the salt-sensitive phenotype of yeast strain G19 (Deltaena1-4), which lacks a major component of Na(+) efflux. It also suppressed a K(+)-transport-defective phenotype of yeast strain CY162 (Deltatrk1Deltatrk2), suggesting the enhancement of K(+) uptake by OsKAT1. By the expression of OsKAT1, the K(+) contents of salt-stressed G19 cells increased during the exponential growth phase. At the linear phase, however, OsKAT1-expressing G19 cells accumulated less Na(+) than nonexpressing cells, but almost the same K(+). The cellular Na(+) to K(+) ratio of OsKAT1-expressing G19 cells remained lower than nonexpressing cells under saline conditions. Rice cells overexpressing OsKAT1 also showed enhanced salt tolerance and increased cellular K(+) content. These functions of OsKAT1 are likely to be common among Shaker K(+) channels because OsAKT1 and Arabidopsis (Arabidopsis thaliana) KAT1 were able to complement the salt-sensitive phenotype of G19 as well as OsKAT1. The expression of OsKAT1 was restricted to internodes and rachides of wild-type rice, whereas other Shaker family genes were expressed in various organs. These results suggest that OsKAT1 is involved in salt tolerance of rice in cooperation with other K(+) channels by participating in maintenance of cytosolic cation homeostasis during salt stress and thus protects cells from Na(+).
Authors:
Toshihiro Obata; Hiroko K Kitamoto; Atsuko Nakamura; Atsunori Fukuda; Yoshiyuki Tanaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-22
Journal Detail:
Title:  Plant physiology     Volume:  144     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-09     Completed Date:  2007-11-30     Revised Date:  2010-09-16    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1978-85     Citation Subset:  IM    
Affiliation:
Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AK100739;  AK120308
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MeSH Terms
Descriptor/Qualifier:
Alkalies / metabolism
Amino Acid Sequence
Arabidopsis / genetics
Cations / metabolism
Cell Line
Gene Expression
Genetic Complementation Test
Homeostasis / physiology
Molecular Sequence Data
Mutation
Oryza sativa / genetics,  growth & development,  metabolism*
Potassium / metabolism*
Saccharomyces cerevisiae / genetics
Shaker Superfamily of Potassium Channels / genetics,  metabolism*
Sodium / metabolism*
Sodium Chloride / metabolism*
Chemical
Reg. No./Substance:
0/Alkalies; 0/Cations; 0/Shaker Superfamily of Potassium Channels; 7440-09-7/Potassium; 7440-23-5/Sodium; 7647-14-5/Sodium Chloride
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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