Document Detail


Rice proteomic analysis: sample preparation for protein identification.
MedLine Citation:
PMID:  23135851     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Rice is one of the most important food and cereal crop plants in the world. Rice proteomics began in the 1990s. Since then, considerable progress has been made in establishing protocols from isolation of rice proteins from different tissues, organs, and organelles, to separation of complex proteins and to their identification by mass spectrometry. Since the year 2000, global proteomics studies have been performed during growth and development under numerous biotic and abiotic environmental conditions. Two-dimensional (2-D) gel-based proteomics platform coupled with mass spectrometry has been retained as the workhorse for proteomics of a variety of rice samples. In this chapter, we describe in detail the different protocols used for isolation of rice proteins, their separation, detection, and identification using gel-based proteomics and mass spectrometry approaches.
Authors:
Ganesh Kumar Agrawal; Nam-Soo Jwa; Young-Ho Jung; Sun Tae Kim; Dea-Wook Kim; Kyoungwon Cho; Junko Shibato; Randeep Rakwal
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  956     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  151-84     Citation Subset:  IM    
Affiliation:
Research Laboratory for Biotechnology and Biochemistry, Kathmandu, Nepal.
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