| Ribozyme targeting of receptor for advanced glycation end products in mouse mesangial cells. | |
| | |
MedLine Citation:
|
PMID: 9571198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Accumulation of extracellular matrix is a characteristic of diabetic nephropathy, and advanced glycation end products (AGEs) are considered to play an important role in the mechanism. To investigate the involvement of the receptor for AGE (RAGE) in upregulation of type IV collagen by AGEs, we applied the hammerhead ribozyme for targeting RAGE. We established a stable mouse mesangial cell line that produces the RAGE-specific ribozyme (Rz-RAGE). Both the RAGE mRNA and protein were decreased in the cell line. The amount of type IV collagen mRNA increased by AGEs' treatment in control cells. In contrast, the increase of type IV collagen induced by AGEs was not observed in the Rz-RAGE-producing cells. We conclude that the induction of type IV collagen by AGEs is mediated by RAGE and this mechanism could be involved in diabetic nephropathy. This study also suggested the experimental/therapeutic potential of hammerhead ribozymes. |
| | |
Authors:
|
H Tsuji; N Iehara; T Masegi; M Imura; J Ohkawa; H Arai; K Ishii; T Kita; T Doi |
Related Documents
:
|
8215988 - Cultural and morphological characteristics of human periodontal ligament cells in vitro. 2632278 - Growth factors as probes of cell aging. 15243128 - Immortalization and characterization of human myometrial cells from term-pregnant patie... 18535488 - Changes in cell-cycle kinetics responsible for limiting somatic growth in mice. 20568698 - Hydrogels based on dual curable chitosan-graft-polyethylene glycol-graft-methacrylate: ... 8215988 - Cultural and morphological characteristics of human periodontal ligament cells in vitro. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 245 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1998 Apr |
Date Detail:
|
Created Date: 1998-06-02 Completed Date: 1998-06-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 583-8 Citation Subset: IM |
Affiliation:
|
Department of Geriatric Medicine, Faculty of Medicine, Kyoto University, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Cell Line Cloning, Molecular Collagen / metabolism Diabetic Nephropathies / etiology Gene Expression Regulation / genetics Glomerular Mesangium / metabolism* Glycosylation End Products, Advanced / pharmacology Immunohistochemistry Mice Molecular Sequence Data RNA, Catalytic / metabolism*, therapeutic use RNA, Messenger / metabolism Receptors, Immunologic / genetics, metabolism* Serum Albumin, Bovine / pharmacology Substrate Specificity Transfection / genetics Up-Regulation / physiology |
| Chemical | |
Reg. No./Substance:
|
0/Glycosylation End Products, Advanced; 0/RNA, Catalytic; 0/RNA, Messenger; 0/Receptors, Immunologic; 0/Serum Albumin, Bovine; 0/advanced glycation end products-bovine serum albumin; 0/advanced glycosylation end-product receptor; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The rfb genes in Azotobacter vinelandii are arranged in a rfbFGC gene cluster: a significant deviati...
Next Document: Molecular cloning of a DEG/ENaC sodium channel cDNA from human testis.