| Ribozyme stability, exon skipping, and a potential role for RNA helicase in group I intron splicing by Coxiella burnetii. | |
| | |
MedLine Citation:
|
PMID: 21803999 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The 23S rRNA gene of Coxiella burnetii, the agent of Q fever in humans, contains an unusually high number of conserved, selfish genetic elements, including two group I introns, termed Cbu.L1917 (L1917) and Cbu.L1951 (L1951). To better understand the role that introns play in Coxiella's biology, we determined the intrinsic stability time periods (in vitro half-lives) of the encoded ribozymes to be ∼15 days for L1917 and ∼5 days for L1951, possibly due to differences in their sizes (551 and 1,559 bases, respectively), relative degrees of compactness of the respective RNA structures, and amounts of single-stranded RNA. In vivo half-lives for both introns were also determined to be ∼11 min by the use of RNase protection assays and an Escherichia coli model. Intron RNAs were quantified in synchronous cultures of C. burnetii and found to closely parallel those of 16S rRNA; i.e., ribozyme levels significantly increased between days 0 and 3 and then remained stable until 8 days postinfection. Both 16S rRNA and ribozyme levels fell during the stationary and death phases (days 8 to 14). The marked stability of the Coxiella intron RNAs is presumably conferred by their association with ribosomes, a stoichiometric relationship that was determined to be one ribozyme, of either type, per 500 ribosomes. Inaccuracies in splicing (exon 2 skipping) were found to increase during the first 5 days in culture, with a rate of approximately one improperly spliced 23S rRNA per 1.3 million copies. The in vitro efficiency of L1917 intron splicing was significantly enhanced in the presence of a recombinant Coxiella RNA DEAD-box helicase (CBU_0670) relative to that of controls, suggesting that this enzyme may serve as an intron RNA splice facilitator in vivo. |
| | |
Authors:
|
Linda D Hicks; Indu Warrier; Rahul Raghavan; Michael F Minnick |
Related Documents
:
|
8300559 - Cloning and recombinant expression of a novel human low molecular weight ca(2+)-depende... 21598019 - The functional rna domain 5bsl3.2 within the ns5b coding sequence influences hepatitis ... 17076249 - Expressed sequence tags from feline uterine library. 2001399 - The cdna sequence of a neutral horseradish peroxidase. 8504829 - Isolation and characterization of a cdna clone encoding a pleurodeles lectin. 2004759 - Human thymosin-beta 4/6-26 gene is part of a multigene family composed of seven members... 23282199 - Optimizing hybrid assembly of next-generation sequence data from enterococcus faecium: ... 1848649 - Cloning of the two essential yeast genes, prp6 and prp9, and their rapid mapping, disru... 8300559 - Cloning and recombinant expression of a novel human low molecular weight ca(2+)-depende... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-07-29 |
Journal Detail:
|
Title: Journal of bacteriology Volume: 193 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-09-14 Completed Date: 2011-11-14 Revised Date: 2013-01-23 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
|
Languages: eng Pagination: 5292-9 Citation Subset: IM |
Affiliation:
|
Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Coxiella burnetii
/
enzymology,
genetics,
metabolism* Exons / genetics* Introns / genetics* Polymerase Chain Reaction RNA Helicases / genetics, metabolism* RNA Splicing / genetics, physiology* RNA Stability / genetics, physiology RNA, Catalytic / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
P20 GM103546/GM/NIGMS NIH HHS; R21 AI078125/AI/NIAID NIH HHS; R21 AI078125/AI/NIAID NIH HHS; R21 AI078125-01A1/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Catalytic; EC 2.7.7.-/RNA Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Choline uptake in Agrobacterium tumefaciens by the high-affinity ChoXWV transporter.
Next Document: UDP-glucuronic acid decarboxylases of Bacteroides fragilis and their prevalence in bacteria.