Document Detail


Ribonucleotide reductase of shrimp white spot syndrome virus (WSSV): expression and enzymatic activity in a baculovirus/insect cell system and WSSV-infected shrimp.
MedLine Citation:
PMID:  12504569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infection of shrimp cells with white spot syndrome virus (WSSV) results in an increase in ribonucleotide reductase (RR) expression at the RNA level. In this article we further express and characterize the induction of a novel ribonucleotide reductase after WSSV infection of shrimp cells. A baculovirus/insect system was used to express the two recombinant protein subunits RR1 and RR2, and a DNA polymerase coupled RR activity assay showed a marked increase in ribonucleotide reductase activity when cell extracts containing recombinant RR1 and RR2 were combined. The same assay revealed that RR activity increased as infection advanced in the gills of experimentally infected shrimp. An increase in RR expression was also detected at the protein level in WSSV-infected shrimp cells. An immunocytochemistry assay by confocal laser scanning microscopy showed that in hemocytes collected from WSSV-infected shrimp, both of the subunit proteins (RR1 and RR2) were concentrated mainly around the nucleus, but only RR1 was detected inside it. All of these results suggest that WSSV RR is functionally involved during WSSV infection.
Authors:
Shinn-Tsuen Lin; Yun-Shiang Chang; Han-Ching Wang; Huey-Fen Tzeng; Zee-Fen Chang; Jung-Yaw Lin; Chung-Hsiung Wang; Chu-Fang Lo; Guang-Hsiung Kou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  304     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-30     Completed Date:  2003-05-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  282-90     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Elsevier Science (USA)
Affiliation:
Department of Zoology, National Taiwan University, Taipei, Taiwan, Republic of China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF332903;  AF369029;  AF440570
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Blotting, Western
DNA Viruses / enzymology*
Decapoda (Crustacea) / virology*
Microscopy, Confocal
Molecular Sequence Data
Nucleopolyhedrovirus / genetics*
Precipitin Tests
Recombinant Proteins / biosynthesis
Ribonucleotide Reductases / analysis,  metabolism*
Spodoptera
Stomach / enzymology,  virology
Chemical
Reg. No./Substance:
0/Recombinant Proteins; EC 1.17.4.-/Ribonucleotide Reductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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