Document Detail

Ribonucleotide reductase in ascites tumour cells detected by electron paramagnetic resonance spectroscopy.
MedLine Citation:
PMID:  3000364     Owner:  NLM     Status:  MEDLINE    
Tyrosine radicals localized in the M2 subunits of ribonucleotide reductase have been detected by electron paramagnetic resonance (EPR) in ordinary ascites tumour cells. The intensity of its doublet EPR spectrum is higher in rapidly proliferating cells. Hydroxyurea, a specific inhibitor of this enzyme, decreases the concentration of the tyrosine radical. Whereas in different ascites tumours the doublet EPR spectrum dominates at g = 2.004, in solid tumours another more intense EPR spectrum from nitrosyl-hemoproteins appears. In conclusion, EPR spectroscopy can be used to monitor the content and variations of active M2 subunits of ribonucleotide reductase in intact ascites tumour cells.
G Lassmann; B Liermann; W Lehmann; H Graetz; A Koberling; P Langen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  132     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1986-01-08     Completed Date:  1986-01-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1137-43     Citation Subset:  IM    
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MeSH Terms
Carcinoma, Ehrlich Tumor / enzymology
Electron Spin Resonance Spectroscopy
Neoplasms, Experimental / enzymology*
Ribonucleotide Reductases / analysis*
Tyrosine / analysis
Reg. No./Substance:
55520-40-6/Tyrosine; EC 1.17.4.-/Ribonucleotide Reductases

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