Document Detail

Ribonucleic acid, deoxyribonucleic acid, and protein content of cells of different ages of Mycobacterium tuberculosis and the ralationship to immunogenicity.
MedLine Citation:
PMID:  4966539     Owner:  NLM     Status:  MEDLINE    
The amount of ribonucleic acid (RNA), protein, and deoxyribonucleic acid (DNA) was determined in pellicle cultures of different ages of the H37Ra strain of Mycobacterium tuberculosis, grown on a synthetic medium. We found that the highest content of RNA and protein was present in 2-week-old cultures, indicating that these cells were in the logarithmic phase of growth. DNA content was highest at 1 and 2 weeks. The amount of all three compounds then decreased about 50% during the following 6 weeks. Two-week-old cells should therefore be used for preparation of the immunogenic ribosomal fraction. The optimal concentration of zinc chloride increased RNA and protein synthesis, and also improved the appearance of the pellicle growth. Two-week-old cells, which contained the largest amount of RNA and protein, immunized mice significantly better than older cells. Since protein and DNA are not involved in the production of immunity, a correlation could be made between amount of RNA and the capacity of viable H37Ra cells to immunize mice. The immunizing capacity of these cells was not affected by ribonuclease, probably because the ribonuclease did not penetrate into the whole cells.
A S Youmans; G P Youmans
Related Documents :
10200269 - Oligoribonuclease is an essential component of the mrna decay pathway.
106059 - Regulation of rna synthesis in tetrahymena pyriformis: secretion of regulatory factors.
366609 - Methionine analogs and cell division regulation in the yeast saccharomyces cerevisiae.
12213489 - Spermine increases phosphatidylinositol 4,5-bisphosphate content in permeabilized and n...
22916109 - Activation of the canonical bone morphogenetic protein (bmp) pathway during lung morpho...
3021099 - Primary isolation and identification of avian rotaviruses from turkeys exhibiting signs...
22078269 - Muc1 mucin is expressed on human t-regulatory cells: function in both co-stimulation an...
21300049 - Cooperative functions of hes/hey genes in auditory hair cell and supporting cell develo...
16324199 - Antibodies targeting p63 react specifically in the cytoplasm of breast epithelial cells...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  95     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1968 Feb 
Date Detail:
Created Date:  1968-05-03     Completed Date:  1968-05-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  272-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacterial Proteins / analysis*
DNA, Bacterial / analysis*
Mycobacterium tuberculosis / analysis*,  growth & development,  immunology*
RNA, Bacterial / analysis*,  pharmacology
Zinc / pharmacology
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/RNA, Bacterial; 7440-66-6/Zinc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Chemical and immunological studies on mycobacterial polysaccharides. 1. Purification and properties ...
Next Document:  Induced sensitization of normal laboratory animals to Brucella abortus endotoxin.