Document Detail


Ribonuclease S redux.
MedLine Citation:
PMID:  21079871     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The S-peptide and S-protein components of bovine pancreatic ribonuclease form a noncovalent complex with restored ribonucleolytic activity. Although this archetypal protein-fragment complementation system has been the object of historic work in protein chemistry, intrinsic limitations compromise its utility. Modern methods are shown to overcome those limitations and enable new applications.
Authors:
Rex W Watkins; Ulrich Arnold; Ronald T Raines
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-11-16
Journal Detail:
Title:  Chemical communications (Cambridge, England)     Volume:  47     ISSN:  1364-548X     ISO Abbreviation:  Chem. Commun. (Camb.)     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-24     Completed Date:  2011-04-11     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  9610838     Medline TA:  Chem Commun (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  973-5     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706-1544, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacillus subtilis / enzymology*
Cattle
Dexfenfluramine
Models, Molecular
Peptide Fragments / chemistry,  metabolism
Protein Conformation
Ribonuclease, Pancreatic / chemistry*,  metabolism*
Subtilisin / metabolism*
Grant Support
ID/Acronym/Agency:
R01 CA073808/CA/NCI NIH HHS; R01 CA073808-14/CA/NCI NIH HHS; R01 GM044783/GM/NIGMS NIH HHS; R01 GM044783-20/GM/NIGMS NIH HHS; T32 GM008505/GM/NIGMS NIH HHS; T32 GM008505-18/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Peptide Fragments; 3239-44-9/Dexfenfluramine; EC 3.1.27.5/Ribonuclease, Pancreatic; EC 3.4.21.62/Subtilisin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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